PMID- 15380940
OWN - NLM
STAT- MEDLINE
DCOM- 20051103
LR  - 20161124
IS  - 0165-0378 (Print)
IS  - 0165-0378 (Linking)
VI  - 63
IP  - 2
DP  - 2004 Oct
TI  - Hypoxia does not reduce HLA-G expression on extravillous cytotrophoblasts.
PG  - 85-95
AB  - Placental hypoxia following the immature remodeling of spiral arteries by 
      extravillous cytotrophoblasts (CTs) is focused on the pathogenesis of 
      pre-eclampsia. At the same time, the expression of human leukocyte antigen 
      (HLA)-G is decreased at the protein and mRNA levels in the pre-eclamptic 
      placenta. In view of the potential function of HLA-G in immunological tolerance 
      in the feto-maternal interface, we were much concerned to find whether the 
      lowered expression of HLA-G in the pre-eclamptic placenta is a precursor or the 
      result of placental hypoxia. The effect of oxygen on the expression of 
      membrane-bound (mb) and soluble (s) HLA-G was investigated in primary cultures of 
      extravillous CTs. The undifferentiated CTs isolated from the first-trimester 
      placenta were cultured with different concentrations of oxygen (20%, 8% and 2%). 
      The protein expression of mbHLA-G and of sHLA-G was assessed using flow 
      cytometry, and mRNA expression was analyzed using real-time PCR. Expression of 
      mbHLA-G and of sHLA-G protein was intensified with time in culture regardless of 
      the oxygen concentration, and the expression intensities were synchronized 
      between the 20% and the 2% oxygen concentrations at each time point. The mRNA 
      expressions of mbHLA-G1 and sHLA-G1 at 2% oxygen were increased to twice those 
      with 20% oxygen. Our findings demonstrate that no reduction of HLA-G was induced 
      in CTs by short-term exposure to hypoxia, although further study may be required 
      to find the effect of chronic hypoxia.
FAU - Nagamatsu, Takeshi
AU  - Nagamatsu T
AD  - Department of Obstetrics and Gynecology, Faculty of Medicine, University of 
      Tokyo, Hongo, Bunkyo-ku, 113-8655 Tokyo, Japan.
FAU - Fujii, Tomoyuki
AU  - Fujii T
FAU - Yamashita, Takahiro
AU  - Yamashita T
FAU - Miki, Akinori
AU  - Miki A
FAU - Kanai, Takao
AU  - Kanai T
FAU - Kusumi, Maki
AU  - Kusumi M
FAU - Osuga, Yutaka
AU  - Osuga Y
FAU - Kozuma, Shiro
AU  - Kozuma S
FAU - Taketani, Yuji
AU  - Taketani Y
LA  - eng
PT  - Journal Article
PL  - Ireland
TA  - J Reprod Immunol
JT  - Journal of reproductive immunology
JID - 8001906
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Cells, Cultured
MH  - *Gene Expression Regulation
MH  - HLA Antigens/genetics/*metabolism
MH  - HLA-G Antigens
MH  - Histocompatibility Antigens Class I/genetics/*metabolism
MH  - Humans
MH  - Hypoxia/*metabolism/pathology
MH  - RNA, Messenger/genetics/metabolism
MH  - Trophoblasts/*metabolism/pathology
EDAT- 2004/09/24 05:00
MHDA- 2005/11/04 09:00
CRDT- 2004/09/24 05:00
PHST- 2004/07/12 00:00 [revised]
PHST- 2004/07/14 00:00 [accepted]
PHST- 2004/09/24 05:00 [pubmed]
PHST- 2005/11/04 09:00 [medline]
PHST- 2004/09/24 05:00 [entrez]
AID - S0165-0378(04)00119-6 [pii]
AID - 10.1016/j.jri.2004.07.001 [doi]
PST - ppublish
SO  - J Reprod Immunol. 2004 Oct;63(2):85-95. doi: 10.1016/j.jri.2004.07.001.