PMID- 15381287
OWN - NLM
STAT- MEDLINE
DCOM- 20050304
LR  - 20141120
IS  - 0306-4522 (Print)
IS  - 0306-4522 (Linking)
VI  - 128
IP  - 3
DP  - 2004
TI  - 5-hydroxytryptamine1A-like receptor activation in the bed nucleus of the stria 
      terminalis: electrophysiological and behavioral studies.
PG  - 583-96
AB  - The anteriorlateral bed nucleus of the stria terminalis (BNST AL) and the 
      serotonergic system are believed to modulate behavioral responses to stressful 
      and/or anxiogenic stimuli. However, although the BNST AL receives heavy 
      serotonergic innervation, the functional significance of this input is not known. 
      Data obtained from in vitro whole-cell patch clamp recording in the rat BNST 
      slice show that exogenous application of 5-hydroxytryptamine (5-HT) evoked a 
      heterogeneous response in BNST AL neurons. The principal action of 5-HT in this 
      region was inhibitory, evoking a membrane hyperpolarization (5-HTHyp) and a 
      concomitant reduction in input resistance in the majority of neurons tested. The 
      broad-spectrum 5-HT1 agonist, 5-carboxamindotryptamine (5-CT), but not 
      R(+/-)8-hydroxydipropylaminotetralin hydrobromide (8-OH-DPAT), mimicked the 
      5-HTHyp response in the BNST. Moreover, the outward current mediating 5-HTHyp was 
      inwardly rectifying and sensitive to the G protein activated inwardly rectifying 
      K+ (G IRK) channel blocker, tertiapin-Q. In the CNS 5-HT1A receptors are thought 
      to couple to GIRK channels, suggesting that 5-HTHyp in BNST AL neurons was 
      mediated by activation of 5-HT1A-like receptors. This was confirmed by the 
      blockade of both 5-HTHyp and 5-CTHyp by the specific 5-HT1A receptor antagonist 
      N-[2-[4-(2-methoxyphenyl)-1-piperazinyl]ethyl]-N-2-pyridinylcyclohexanecarboxamide 
      maleate salt (WAY100635 200nM). Furthermore, an in vivo examination of the 
      functional consequences of 5-HT1A-like induced inhibition of BNST neurons 
      revealed that infusion of 5-CT into the BNST significantly reduced the acoustic 
      startle response, without affecting the general motor activity of the animals. 
      These data point to the possibility that 5-HT1A mediated inhibition of the BNST 
      AL could contribute to an anxiolytic action. Hence, we propose that in response 
      to stressful stimuli, enhanced levels of 5-HT in the BNST AL plays a critical 
      homeostatic role in feedback inhibition of the anxiogenic response to these 
      stimuli.
FAU - Levita, L
AU  - Levita L
AD  - Department of Psychiatry and Behavioral Neuroscience, Emory University, Yerkes 
      Neuroscience Building, 954 Gatewood Drive, Room 5220, Atlanta, GA 30322, USA.
FAU - Hammack, S E
AU  - Hammack SE
FAU - Mania, I
AU  - Mania I
FAU - Li, X-Y
AU  - Li XY
FAU - Davis, M
AU  - Davis M
FAU - Rainnie, D G
AU  - Rainnie DG
LA  - eng
GR  - MH 57250/MH/NIMH NIH HHS/United States
GR  - MH47840/MH/NIMH NIH HHS/United States
GR  - MH59906/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (G Protein-Coupled Inwardly-Rectifying Potassium Channels)
RN  - 0 (Potassium Channel Blockers)
RN  - 0 (Potassium Channels, Inwardly Rectifying)
RN  - 0 (Serotonin 5-HT1 Receptor Agonists)
RN  - 0 (Serotonin Antagonists)
RN  - 0 (Serotonin Receptor Agonists)
RN  - 112692-38-3 (Receptor, Serotonin, 5-HT1A)
RN  - 333DO1RDJY (Serotonin)
SB  - IM
MH  - Afferent Pathways/drug effects/*metabolism
MH  - Animals
MH  - G Protein-Coupled Inwardly-Rectifying Potassium Channels
MH  - Male
MH  - Membrane Potentials/drug effects/physiology
MH  - Neural Inhibition/drug effects/physiology
MH  - Neurons/drug effects/*metabolism
MH  - Organ Culture Techniques
MH  - Patch-Clamp Techniques
MH  - Potassium Channel Blockers/pharmacology
MH  - Potassium Channels, Inwardly Rectifying/drug effects/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, Serotonin, 5-HT1A/*metabolism
MH  - Reflex, Startle/drug effects/physiology
MH  - Septal Nuclei/drug effects/*metabolism
MH  - Serotonin/*metabolism/pharmacology
MH  - Serotonin 5-HT1 Receptor Agonists
MH  - Serotonin Antagonists/pharmacology
MH  - Serotonin Receptor Agonists/pharmacology
EDAT- 2004/09/24 05:00
MHDA- 2005/03/05 09:00
CRDT- 2004/09/24 05:00
PHST- 2004/06/23 00:00 [accepted]
PHST- 2004/09/24 05:00 [pubmed]
PHST- 2005/03/05 09:00 [medline]
PHST- 2004/09/24 05:00 [entrez]
AID - S0306-4522(04)00532-9 [pii]
AID - 10.1016/j.neuroscience.2004.06.037 [doi]
PST - ppublish
SO  - Neuroscience. 2004;128(3):583-96. doi: 10.1016/j.neuroscience.2004.06.037.