PMID- 15381671
OWN - NLM
STAT- MEDLINE
DCOM- 20050324
LR  - 20081121
IS  - 0953-8178 (Print)
IS  - 0953-8178 (Linking)
VI  - 16
IP  - 11
DP  - 2004 Nov
TI  - Mucin-1 is expressed on dendritic cells, both in vitro and in vivo.
PG  - 1561-71
AB  - Dendritic cells (DCs) are the best professional antigen-presenting cells to 
      stimulate cytotoxic as well as T helper cells and are therefore appropriate 
      candidates for establishing immunotherapy. The concept of our vaccination program 
      is to introduce the tumor-associated antigen mucin-1 (MUC1) into DCs. Analysis of 
      immature and mature DCs--before transducing the antigen MUC1--already 
      demonstrated expression of MUC1 on in vitro monocyte-derived DCs upon maturation. 
      Different culture methods as well as maturation cocktails showed similar results 
      concerning the upregulation of MUC1 expression. Furthermore, we studied the 
      expression of MUC1 on DCs in vivo. No MUC1 expression was found on blood DCs, or 
      on thymic or tonsil DCs. On the other hand, synovial fluid from patients with 
      arthritis contained DCs that were found to express MUC1. This study shows for the 
      first time that the tumor-associated antigen MUC1 is expressed on in vivo DCs. We 
      further show that MUC1 is also expressed on in vitro cultured bone marrow-derived 
      DCs of human MUC1 transgenic mice, supporting the relevance of this mouse model 
      to the human situation. The observation that MUC1 is present on in vivo DCs 
      suggests a functional role, but this physiological function remains to be 
      elucidated.
FAU - Cloosen, Silvie
AU  - Cloosen S
AD  - Department of Internal Medicine, Division of Hemato-Oncology, University Hospital 
      Maastricht, PO Box 5800, 6202 AZ Maastricht, The Netherlands. 
      S.Cloosen@immuno.unimaas.nl
FAU - Thio, Marco
AU  - Thio M
FAU - Vanclee, Ariane
AU  - Vanclee A
FAU - van Leeuwen, Ellen B M
AU  - van Leeuwen EB
FAU - Senden-Gijsbers, Birgit L M G
AU  - Senden-Gijsbers BL
FAU - Oving, Ellis B H
AU  - Oving EB
FAU - Germeraad, Wilfred T V
AU  - Germeraad WT
FAU - Bos, Gerard M J
AU  - Bos GM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20040920
PL  - England
TA  - Int Immunol
JT  - International immunology
JID - 8916182
RN  - 0 (Mucin-1)
SB  - IM
MH  - Animals
MH  - Antigen Presentation/immunology
MH  - Cells, Cultured
MH  - Dendritic Cells/*immunology/transplantation
MH  - Gene Expression Regulation/*immunology
MH  - Humans
MH  - Immunotherapy, Adoptive
MH  - Mice
MH  - Mice, Transgenic
MH  - Mucin-1/genetics/*immunology
MH  - Neoplasms/pathology/therapy
MH  - T-Lymphocytes, Cytotoxic/*immunology
MH  - T-Lymphocytes, Helper-Inducer/*immunology
EDAT- 2004/09/24 05:00
MHDA- 2005/03/25 09:00
CRDT- 2004/09/24 05:00
PHST- 2004/09/24 05:00 [pubmed]
PHST- 2005/03/25 09:00 [medline]
PHST- 2004/09/24 05:00 [entrez]
AID - dxh157 [pii]
AID - 10.1093/intimm/dxh157 [doi]
PST - ppublish
SO  - Int Immunol. 2004 Nov;16(11):1561-71. doi: 10.1093/intimm/dxh157. Epub 2004 Sep 
      20.