PMID- 15381691
OWN - NLM
STAT- MEDLINE
DCOM- 20050207
LR  - 20220224
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 279
IP  - 48
DP  - 2004 Nov 26
TI  - Nuclear factor 1 and octamer transcription factor 1 binding preset the chromatin 
      structure of the mouse mammary tumor virus promoter for hormone induction.
PG  - 49857-67
AB  - When the mouse mammary tumor virus (MMTV) is integrated into the genome of a 
      mammalian cell, its long terminal repeat (LTR) harbors six specifically 
      positioned nucleosomes. Transcription from the MMTV promoter is regulated by the 
      glucocorticoid hormone via the glucocorticoid receptor (GR). The mechanism of the 
      apparently constitutive nucleosome arrangement has remained unclear. Previous in 
      vitro reconstitution of nucleosome(s) on small segments of the MMTV LTR suggested 
      that the DNA sequence was decisive for the nucleosome arrangement. However, 
      microinjection of MMTV LTR DNA in Xenopus oocytes rendered randomly distributed 
      nucleosomes. This indicated that oocytes lack factor(s) that induces nucleosome 
      positioning at the MMTV LTR in other cells. Here we demonstrate that specific and 
      concomitant binding of nuclear factor 1 (NF1) and octamer factor 1 (Oct1) to 
      their cognate sites within the MMTV promoter induce a partial nucleosome 
      positioning that is an intermediary state between the randomly organized inactive 
      promoter and the hormone and GR-activated promoter containing distinctly 
      positioned nucleosomes. Oct1 and NF1 reciprocally facilitate each other's binding 
      to the MMTV LTR in vivo. The NF1 and Oct1 binding also facilitate 
      hormone-dependent GR-DNA interaction and result in a faster and stronger hormone 
      response. Since NF1 and Oct1 generate an intermediary state of nucleosome 
      positioning and enhance the hormone-induced response, we refer to this as a 
      preset chromatin structure. We propose that this state of NF1 and Oct1-induced 
      chromatin presetting mimics the early step(s) of chromatin remodeling involved in 
      tissue-specific gene expression.
FAU - Belikov, Sergey
AU  - Belikov S
AD  - Department of Cell and Molecular Biology, The Medical Nobel Institute, P. O. Box 
      285, Karolinska Institutet, Stockholm SE-17177, Sweden.
FAU - Holmqvist, Per-Henrik
AU  - Holmqvist PH
FAU - Astrand, Carolina
AU  - Astrand C
FAU - Wrange, Orjan
AU  - Wrange O
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20040920
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (CCAAT-Enhancer-Binding Proteins)
RN  - 0 (Chromatin)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Hormones)
RN  - 0 (NFI Transcription Factors)
RN  - 0 (Nucleosomes)
RN  - 0 (Octamer Transcription Factor-1)
RN  - 0 (POU2F1 protein, Xenopus)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factors)
RN  - 0 (Xenopus Proteins)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - CCAAT-Enhancer-Binding Proteins/*metabolism
MH  - Chromatin/*metabolism
MH  - DNA-Binding Proteins/*metabolism
MH  - Hormones/*metabolism
MH  - Mammary Tumor Virus, Mouse/genetics/*metabolism
MH  - Molecular Sequence Data
MH  - NFI Transcription Factors
MH  - Nucleosomes/metabolism
MH  - Octamer Transcription Factor-1
MH  - Promoter Regions, Genetic
MH  - RNA, Messenger/metabolism
MH  - Transcription Factors/*metabolism
MH  - Transcription, Genetic/physiology
MH  - Xenopus
MH  - Xenopus Proteins
EDAT- 2004/09/24 05:00
MHDA- 2005/02/08 09:00
CRDT- 2004/09/24 05:00
PHST- 2004/09/24 05:00 [pubmed]
PHST- 2005/02/08 09:00 [medline]
PHST- 2004/09/24 05:00 [entrez]
AID - S0021-9258(20)67772-9 [pii]
AID - 10.1074/jbc.M409713200 [doi]
PST - ppublish
SO  - J Biol Chem. 2004 Nov 26;279(48):49857-67. doi: 10.1074/jbc.M409713200. Epub 2004 
      Sep 20.