PMID- 15385579
OWN - NLM
STAT- MEDLINE
DCOM- 20041102
LR  - 20220129
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 52
IP  - 10
DP  - 2004 Oct
TI  - Improvement of combined FISH and immunofluorescence to trace the fate of somatic 
      stem cells after transplantation.
PG  - 1333-9
AB  - Fluorescence in situ hybridization (FISH) combined with immunohistochemistry of 
      tissue-specific markers provides a reliable method for characterizing the fate of 
      somatic stem cells in transplantation experiments. Furthermore, the association 
      between FISH and fluorescent gene reporter detection can unravel cell fusion 
      phenomena, which could account for apparent transdifferentiation events. However, 
      despite the widespread use of these techniques, they still require 
      labor-extensive protocol adjustments to achieve correct and satisfactory 
      simultaneous signal detection. In the present paper, we describe an improvement 
      of simultaneous FISH and immunofluorescence detection. We applied this protocol 
      to the identification of transplanted human and mouse hematopoietic stem cells in 
      murine brain and muscle. This technique provides unique opportunities for 
      following the path taken by transplanted cells and their differentiation into 
      mature cell types.
CI  - Copyright The Histochemical Society, Inc.
FAU - Donadoni, Chiara
AU  - Donadoni C
AD  - Dipartimento di Scienze Neurologiche, Universita di Milano, Padiglione Ponti, 
      Ospedale Policlinico, Via Francesco Sforza 35, 20122 Milan, Italy. 
      giacomo.comi@unimi.it
FAU - Corti, Stefania
AU  - Corti S
FAU - Locatelli, Federica
AU  - Locatelli F
FAU - Papadimitriou, Dimitra
AU  - Papadimitriou D
FAU - Guglieri, Michela
AU  - Guglieri M
FAU - Strazzer, Sandra
AU  - Strazzer S
FAU - Bossolasco, Patrizia
AU  - Bossolasco P
FAU - Salani, Sabrina
AU  - Salani S
FAU - Comi, Giacomo P
AU  - Comi GP
LA  - eng
GR  - GTF01001/TI_/Telethon/Italy
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Bone Marrow Transplantation
MH  - Brain/cytology/surgery/ultrastructure
MH  - Fluorescent Antibody Technique/*methods
MH  - *Hematopoietic Stem Cell Transplantation
MH  - Hematopoietic Stem Cells/*cytology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Muscle, Skeletal/cytology
EDAT- 2004/09/24 05:00
MHDA- 2004/11/04 09:00
CRDT- 2004/09/24 05:00
PHST- 2004/09/24 05:00 [pubmed]
PHST- 2004/11/04 09:00 [medline]
PHST- 2004/09/24 05:00 [entrez]
AID - 52/10/1333 [pii]
AID - 10.1177/002215540405201009 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 2004 Oct;52(10):1333-9. doi: 10.1177/002215540405201009.