PMID- 15386800
OWN - NLM
STAT- MEDLINE
DCOM- 20050408
LR  - 20131121
IS  - 1522-7235 (Print)
IS  - 1522-7235 (Linking)
VI  - 19
IP  - 5
DP  - 2004 Sep-Oct
TI  - Chemiluminescence assay of lipase activity using a synthetic substrate as 
      proenhancer for luminol chemiluminescence reaction.
PG  - 259-64
AB  - A novel chemiluminescence (CL) assay method for lipase (triacylglycerol lipase, 
      E.C.3.1.1.3) activity was developed by using the lauric acid ester of 
      2-(4-hydroxyphenyl)-4,5-diphenylimidazole (HDI) as a substrate. The method is 
      based on the enhanced CL reaction of luminol-hydrogen peroxide-horseradish 
      peroxidase (HRP) with HDI that is liberated from the substrate by enzymatic 
      hydrolysis. To simplify the assay procedure, both the hydrolysis of the substrate 
      and the enhanced CL reaction were performed in the same reaction mixture. Lipases 
      from Candida cylindracea and porcine pancreas were successfully determined with 
      the detection limits (blank signal + 3 SD) of 0.05 and 50.0 mU/tube, 
      respectively. The method is simple and rapid, permitting the completion of single 
      assay within 5 min. The reproducibilities obtained with replicate assays were 
      relative standard deviations (RSDs) of <=> 4.7% for within-day and <=> 6.0% for 
      between-day assays.
FAU - Ichibangase, Tomoko
AU  - Ichibangase T
AD  - Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, 
      Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.
FAU - Ohba, Yoshihito
AU  - Ohba Y
FAU - Kishikawa, Naoya
AU  - Kishikawa N
FAU - Nakashima, Kenichiro
AU  - Nakashima K
FAU - Kuroda, Naotaka
AU  - Kuroda N
LA  - eng
PT  - Journal Article
PL  - England
TA  - Luminescence
JT  - Luminescence : the journal of biological and chemical luminescence
JID - 100889025
RN  - 0 (2-(4-hydroxyphenyl)-4,5-diphenylimidazole)
RN  - 0 (Imidazoles)
RN  - 0 (Triglycerides)
RN  - 5EXP385Q4F (Luminol)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.11.1.- (Horseradish Peroxidase)
RN  - EC 3.1.1.3 (Lipase)
SB  - IM
MH  - Animals
MH  - Candida/enzymology
MH  - Horseradish Peroxidase/chemistry
MH  - Hydrogen Peroxide/chemistry
MH  - Hydrolysis
MH  - Imidazoles/*chemistry
MH  - *Lipase/analysis/chemistry/metabolism
MH  - Luminescent Measurements/*methods
MH  - Luminol/*chemistry
MH  - Pancreas/enzymology
MH  - Sensitivity and Specificity
MH  - Substrate Specificity
MH  - Swine
MH  - Triglycerides/analysis/metabolism
EDAT- 2004/09/24 05:00
MHDA- 2005/04/09 09:00
CRDT- 2004/09/24 05:00
PHST- 2004/09/24 05:00 [pubmed]
PHST- 2005/04/09 09:00 [medline]
PHST- 2004/09/24 05:00 [entrez]
AID - 10.1002/bio.770 [doi]
PST - ppublish
SO  - Luminescence. 2004 Sep-Oct;19(5):259-64. doi: 10.1002/bio.770.