PMID- 15387132
OWN - NLM
STAT- MEDLINE
DCOM- 20041014
LR  - 20090918
IS  - 1477-8920 (Print)
IS  - 1477-8920 (Linking)
VI  - 2
IP  - 2
DP  - 2004 Jun
TI  - Detection, integration and persistence of aeromonads in water distribution pipe 
      biofilms.
PG  - 83-96
AB  - The occurrence of Aeromonas spp. within biofilms formed on stainless steel (SS), 
      unplasticized polyvinyl chloride (uPVC) and glass (GL) substrata was investigated 
      in modified Robbins Devices (MRD) in potable (MRD-p) and recycled (MRD-r) water 
      systems, a Biofilm Reactor (BR) and a laboratory-scale pipe loop (PL) receiving 
      simulated recycled wastewater. No aeromonads were isolated from the MRD-p whereas 
      3-10% of SS and uPVC coupons (mean 3.85 CFU cm(-2) and 12.8 CFU cm(-2), 
      respectively) were aeromonad-positive in the MRD-r. Aeromonads were isolated from 
      six SS coupons (67%) (mean 63.4 CFU cm(-2)) and nine uPVC coupons (100%) (mean 
      6.50x 10(2) CFU cm(-2)) in the BR fed with recycled water and from all coupons 
      (100%) in the simulated recycled water system (PL). Mean numbers of aeromonads on 
      GL and SS coupons were 5.83 x 10(2) CFU cm(-2) and 8.73 x 10(2) CFU cm(-2), 
      respectively. No isolate was of known human health significance (i.e. Aeromonas 
      caviae, A. hydrophila or A. veronii), though they were confirmed as Aeromonas 
      spp. by PCR and fluorescence in situ hybridization (FISH). Challenging the PL 
      biofilms with a slug dose of A. hydrophila (ATCC 14715) showed that biofilm in 
      the PL accumulated in the order of 10(3)-10(4) A. hydrophila cm(-2), the number 
      of which decreased over time, though could not be explained in terms of 
      conventional 1st order decay kinetics. A sub-population of FISH-positive A. 
      hydrophila became established within the biofilm, thereby demonstrating their 
      ability to incorporate and persist in biofilms formed within distribution pipe 
      systems. A similar observation was not made for culturable aeromonads, though the 
      exact human health significance of this remains unknown. These findings, however, 
      further question the adequacy of culture-based techniques and their often 
      anomalous discrepancy with direct techniques for the enumeration of bacterial 
      pathogens in environmental samples.
FAU - Bomo, A-M
AU  - Bomo AM
AD  - Department of Mathematical Sciences and Technology, Agricultural University of 
      Norway, 1432 As, Norway. anne-marie.bomo@imt.nlh.no
FAU - Storey, M V
AU  - Storey MV
FAU - Ashbolt, N J
AU  - Ashbolt NJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Water Health
JT  - Journal of water and health
JID - 101185420
SB  - IM
MH  - *Aeromonas/isolation & purification/physiology
MH  - Aeromonas hydrophila/isolation & purification/physiology
MH  - Analysis of Variance
MH  - *Biofilms/growth & development
MH  - Colony Count, Microbial
MH  - Environmental Monitoring/*methods
MH  - Humans
MH  - Linear Models
MH  - New South Wales
MH  - *Water Supply
EDAT- 2004/09/25 05:00
MHDA- 2004/10/16 09:00
CRDT- 2004/09/25 05:00
PHST- 2004/09/25 05:00 [pubmed]
PHST- 2004/10/16 09:00 [medline]
PHST- 2004/09/25 05:00 [entrez]
PST - ppublish
SO  - J Water Health. 2004 Jun;2(2):83-96.