PMID- 15388788
OWN - NLM
STAT- MEDLINE
DCOM- 20050420
LR  - 20090812
IS  - 0888-8809 (Print)
IS  - 0888-8809 (Linking)
VI  - 19
IP  - 1
DP  - 2005 Jan
TI  - Steroid sulfotransferase 2A1 gene transcription is regulated by steroidogenic 
      factor 1 and GATA-6 in the human adrenal.
PG  - 184-97
AB  - Sulfonation is a phase II conjugation reaction responsible for the 
      biotransformation of many compounds including steroids, bile acids, and drugs. 
      Humans are presently known to express at least five cytosolic sulfotransferase 
      (SULT) enzymes, of which only two are hydroxysteroid SULT, SULT2A1, commonly 
      known as steroid sulfotransferase, and the cholesterol sulfotransferase SULT2B1. 
      SULT2A1 is highly expressed in the adrenal where it is responsible for the 
      sulfation of hydroxysteroids including conversion of dehydroepiandrosterone to 
      dehydroepiandrosterone sulfate and in the liver where it is responsible for 
      sulfation of bile acids and circulating hydroxysteroids. Little is known 
      concerning the transcriptional regulation of human SULT2A1 in adrenal. Herein we 
      demonstrate the role of two transcription factors, steroidogenic factor 1 (SF1) 
      and GATA-6, in the regulation of SULT2A1 transcription. These transcription 
      factors were quantified by real-time RT-PCR in normal human adrenal tissue. 
      Transient transfection assays with deleted and mutated SULT2A1 promoter 
      constructs allowed for the determination of specific SF1 and GATA binding 
      cis-regulatory elements necessary for transactivation of SULT2A1 promoter, and 
      binding was confirmed by EMSA analysis. Both SF1 and GATA-6 were positive 
      regulators of SULT2A1 promoter constructs. These data support the hypothesis that 
      adrenal SULT2A1 expression is regulated by SF1 and GATA-6.
FAU - Saner, Karla J
AU  - Saner KJ
AD  - Department of Obstetrics and Gynecology, University of Texas Southwestern Medical 
      Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390-9032, USA.
FAU - Suzuki, Takashi
AU  - Suzuki T
FAU - Sasano, Hironobu
AU  - Sasano H
FAU - Pizzey, John
AU  - Pizzey J
FAU - Ho, Clement
AU  - Ho C
FAU - Strauss, Jerome F 3rd
AU  - Strauss JF 3rd
FAU - Carr, Bruce R
AU  - Carr BR
FAU - Rainey, William E
AU  - Rainey WE
LA  - eng
GR  - DK069950/DK/NIDDK NIH HHS/United States
GR  - DK43140/DK/NIDDK NIH HHS/United States
GR  - HD-06274/HD/NICHD NIH HHS/United States
GR  - HD11149/HD/NICHD NIH HHS/United States
GR  - T32-HD07190/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20040923
PL  - United States
TA  - Mol Endocrinol
JT  - Molecular endocrinology (Baltimore, Md.)
JID - 8801431
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (GATA6 Transcription Factor)
RN  - 0 (GATA6 protein, human)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (NR5A1 protein, human)
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Steroidogenic Factor 1)
RN  - 0 (Transcription Factors)
RN  - EC 2.8.2.- (Sulfotransferases)
RN  - EC 2.8.2.15 (steroid sulfotransferase)
SB  - IM
MH  - Adrenal Glands/enzymology/*metabolism
MH  - Binding Sites
MH  - Cell Line
MH  - DNA-Binding Proteins/*metabolism
MH  - Electrophoretic Mobility Shift Assay
MH  - GATA6 Transcription Factor
MH  - Gene Expression Regulation/*genetics
MH  - Homeodomain Proteins
MH  - Humans
MH  - Organ Specificity
MH  - Promoter Regions, Genetic/genetics
MH  - Protein Isoforms/genetics/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Receptors, Cytoplasmic and Nuclear
MH  - Response Elements/genetics
MH  - Steroidogenic Factor 1
MH  - Sulfotransferases/*genetics
MH  - Transcription Factors/*metabolism
MH  - Transcription, Genetic/*genetics
MH  - Transcriptional Activation/genetics
EDAT- 2004/09/25 05:00
MHDA- 2005/04/21 09:00
CRDT- 2004/09/25 05:00
PHST- 2004/09/25 05:00 [pubmed]
PHST- 2005/04/21 09:00 [medline]
PHST- 2004/09/25 05:00 [entrez]
AID - me.2003-0332 [pii]
AID - 10.1210/me.2003-0332 [doi]
PST - ppublish
SO  - Mol Endocrinol. 2005 Jan;19(1):184-97. doi: 10.1210/me.2003-0332. Epub 2004 Sep 
      23.