PMID- 15388810
OWN - NLM
STAT- MEDLINE
DCOM- 20050131
LR  - 20131121
IS  - 0267-8357 (Print)
IS  - 0267-8357 (Linking)
VI  - 19
IP  - 5
DP  - 2004 Sep
TI  - Tripterygium hypoglaucum (level) Hutch induces aneuploidy of chromosome 8 in 
      mouse bone marrow cells and sperm.
PG  - 379-82
AB  - Aneuploidy of mouse chromosome 8 induced by a Chinese medicinal herb, 
      Tripterygium hypoglaucum (level) Hutch (THH) was investigated by fluorescence in 
      situ hybridization (FISH) in vivo. Male mice were treated with THH (single i.p. 
      injection) at doses of 120, 240 and 480 mg/kg. Colchicine (COL, 1.5 mg/kg i.p.) 
      was used as a positive control. Bone marrow cells and epididymal sperm were 
      collected 24 h and 22 days after treatment, respectively. Chromosome 8 
      aneuploidies induced by THH in bone marrow cells and sperm were determined by 
      FISH with a biotin-16-dUTP labelled DNA probe corresponding to the centromeric 
      region of chromosome 8. The hybridized probe was detected with avidin-FITC. The 
      frequencies of trisomy 8 in bone marrow cells were 0.16% in the solvent control 
      group, 0.39% in the COL-treated group and 0.33, 0.41 and 0.41% in the THH-treated 
      groups, respectively. The frequencies of disomy 8 sperm were 0.11% in the solvent 
      control group, 0.27% in the COL-treated group and 0.23, 0.27 and 0.27% in the 
      THH-treated groups, respectively. The experiment showed that induced aneuploidy 
      frequencies were higher in bone marrow cells than in sperm with COL and the two 
      higher doses of THH (P < 0.05). All groups were significantly different from the 
      corresponding solvent controls (P < 0.01-0.001), but there was no dose-related 
      increase in either cell type. Considering the present results together with our 
      previous studies, it appears that THH is a potent mammalian aneugen which may 
      pose a genetic risk to human patients.
FAU - Xu, Wang
AU  - Xu W
AD  - State Key Laboratory of Genetic Engineering, Institute of Genetics, School of 
      Life Sciences, Fudan University, Shanghai, 200433, People's Republic of China.
FAU - Ziqing, Liang
AU  - Ziqing L
FAU - Yinrun, Ding
AU  - Yinrun D
FAU - Xiaoyan, Wang
AU  - Xiaoyan W
FAU - Jinglun, Xue
AU  - Jinglun X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mutagenesis
JT  - Mutagenesis
JID - 8707812
RN  - 0 (Aneugens)
RN  - 0 (DNA Primers)
RN  - 0 (Mutagens)
RN  - 0 (Plant Preparations)
RN  - 9007-49-2 (DNA)
RN  - SML2Y3J35T (Colchicine)
SB  - IM
MH  - Aneugens
MH  - *Aneuploidy
MH  - Animals
MH  - Bone Marrow Cells/cytology/*drug effects
MH  - *Chromosomes, Human, Pair 8
MH  - Colchicine/pharmacology
MH  - DNA/chemistry
MH  - DNA Primers/chemistry
MH  - Dose-Response Relationship, Drug
MH  - Epididymis/drug effects
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Medicine, Chinese Traditional/adverse effects
MH  - Mice
MH  - *Mutagens
MH  - Nucleic Acid Hybridization
MH  - Plant Preparations
MH  - Ploidies
MH  - Spermatozoa/*drug effects
MH  - Time Factors
MH  - *Tripterygium
EDAT- 2004/09/25 05:00
MHDA- 2005/02/03 09:00
CRDT- 2004/09/25 05:00
PHST- 2004/09/25 05:00 [pubmed]
PHST- 2005/02/03 09:00 [medline]
PHST- 2004/09/25 05:00 [entrez]
AID - 19/5/379 [pii]
AID - 10.1093/mutage/geh044 [doi]
PST - ppublish
SO  - Mutagenesis. 2004 Sep;19(5):379-82. doi: 10.1093/mutage/geh044.