PMID- 1539113
OWN - NLM
STAT- MEDLINE
DCOM- 19920331
LR  - 20190827
IS  - 0167-0115 (Print)
IS  - 0167-0115 (Linking)
VI  - 37
IP  - 2
DP  - 1992 Jan 23
TI  - Participation of pertussis toxin-sensitive GTP-binding regulatory proteins in the 
      suppression of baroreceptor reflex by neurotensin in the rat.
PG  - 167-80
AB  - We evaluated the molecular mechanism that may underlie the suppressive effect of 
      neurotensin (NT) on the baroreceptor reflex (BRR), using Sprague-Dawley rats that 
      were anesthetized with sodium pentobarbital (50 mg/kg, i.p.). 
      Intracerebroventricular (i.c.v.) application of NT (15 nmol) significantly 
      inhibited the BRR response. Such an inhibition was appreciably antagonized by 
      pretreating animals with i.c.v. injection of pertussis toxin (10 or 20 pmol), 
      N-ethylmaleimide (1 or 2 nmol), forskolin (30 or 60 nmol) or phorbol 12-myristate 
      13-acetate (2 or 4 nmol), but not by cholera toxin (15 or 30 pmol). More 
      specifically, pretreatments with bilateral microinjection into the nucleus 
      tractus solitarius (NTS) of pertussis toxin (80 or 160 fmol), N-ethylmaleimide 
      (80 pmol), forskolin (480 pmol) or phorbol 12-myristate 13-acetate (16 or 32 
      pmol) also blunted the NT-induced suppression of BRR, although cholera toxin (120 
      or 240 fmol), or 1,9-dideoxyforskolin (480 pmol) had no appreciable effect. These 
      results suggest that a pertussis toxin-sensitive guanine nucleotide-binding 
      regulatory protein(s), which is not likely to be Gs, possibly Gi or Gp, may be 
      involved in the transmembrane signaling process that underlies the suppression of 
      BRR response by NT at the NTS.
FAU - Fu, M J
AU  - Fu MJ
AD  - Institute of Pharmacology, National Yang-Ming Medical College, Taipei, Taiwan, 
      R.O.C.
FAU - Lin, K S
AU  - Lin KS
FAU - Chan, J Y
AU  - Chan JY
FAU - Chan, S H
AU  - Chan SH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Regul Pept
JT  - Regulatory peptides
JID - 8100479
RN  - 0 (Virulence Factors, Bordetella)
RN  - 1F7A44V6OU (Colforsin)
RN  - 39379-15-2 (Neurotensin)
RN  - 9012-63-9 (Cholera Toxin)
RN  - EC 2.4.2.31 (Pertussis Toxin)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - O3C74ACM9V (Ethylmaleimide)
RN  - OAW710HWIX (1,9-dideoxyforskolin)
SB  - IM
MH  - Animals
MH  - Cholera Toxin/pharmacology
MH  - Colforsin/administration & dosage/analogs & derivatives/pharmacology
MH  - Ethylmaleimide/pharmacology
MH  - GTP-Binding Proteins/*physiology
MH  - Male
MH  - Neurotensin/administration & dosage/antagonists & inhibitors/*pharmacology
MH  - *Pertussis Toxin
MH  - Pressoreceptors/*drug effects
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Reflex/*drug effects
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Virulence Factors, Bordetella/*pharmacology
EDAT- 1992/01/23 00:00
MHDA- 1992/01/23 00:01
CRDT- 1992/01/23 00:00
PHST- 1992/01/23 00:00 [pubmed]
PHST- 1992/01/23 00:01 [medline]
PHST- 1992/01/23 00:00 [entrez]
AID - 0167-0115(92)90665-H [pii]
AID - 10.1016/0167-0115(92)90665-h [doi]
PST - ppublish
SO  - Regul Pept. 1992 Jan 23;37(2):167-80. doi: 10.1016/0167-0115(92)90665-h.