PMID- 1542695
OWN - NLM
STAT- MEDLINE
DCOM- 19920407
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 89
IP  - 5
DP  - 1992 Mar 1
TI  - Identification of a genetic locus of Haemophilus influenzae type b necessary for 
      the binding and utilization of heme bound to human hemopexin.
PG  - 1973-7
AB  - The mechanism(s) used by Haemophilus influenzae to acquire the essential nutrient 
      heme from its human host has not been elucidated. The heme carried by the 
      high-affinity serum protein hemopexin is one potential source of this 
      micronutrient in vivo. A colony-blot assay revealed that heme-human 
      hemopexin-binding activity was shared among most capsular serotype b strains of 
      H. influenzae but was uncommon among other strains. We have identified a 
      recombinant clone binding heme-human hemopexin from a H. influenzae type b (Hib) 
      genomic library expressed in Escherichia coli. Both the Hib strain and the 
      heme-hemopexin-binding clone expressed a polypeptide of approximately 100 kDa 
      that bound radiolabeled heme-hemopexin. Oligonucleotide linker insertion 
      mutagenesis of the plasmid DNA from this recombinant clone was used to confirm 
      that expression of the 100-kDa protein correlated with the heme-hemopexin-binding 
      activity. Exchange of one of these mutant alleles into the Hib chromosome 
      eliminated expression of both the 100-kDa protein and the heme-hemopexin-binding 
      activity. Furthermore, this Hib mutant was unable to utilize heme-human hemopexin 
      as a heme source.
FAU - Hanson, M S
AU  - Hanson MS
AD  - Department of Microbiology, University of Texas Southwestern Medical Center, 
      Dallas 75235.
FAU - Pelzel, S E
AU  - Pelzel SE
FAU - Latimer, J
AU  - Latimer J
FAU - Muller-Eberhard, U
AU  - Muller-Eberhard U
FAU - Hansen, E J
AU  - Hansen EJ
LA  - eng
GR  - AI-17621/AI/NIAID NIH HHS/United States
GR  - DK-30203/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Recombinant Proteins)
RN  - 42VZT0U6YR (Heme)
RN  - 9013-71-2 (Hemopexin)
SB  - IM
MH  - Bacterial Proteins/chemistry/genetics/*metabolism
MH  - Cloning, Molecular
MH  - DNA, Bacterial/genetics
MH  - *Genes, Bacterial
MH  - Haemophilus influenzae/*genetics/metabolism/pathogenicity
MH  - Heme/*metabolism
MH  - Hemopexin/*metabolism
MH  - Humans
MH  - In Vitro Techniques
MH  - Molecular Weight
MH  - Recombinant Proteins/metabolism
MH  - Restriction Mapping
PMC - PMC48576
EDAT- 1992/03/01 00:00
MHDA- 1992/03/01 00:01
PMCR- 1992/09/01
CRDT- 1992/03/01 00:00
PHST- 1992/03/01 00:00 [pubmed]
PHST- 1992/03/01 00:01 [medline]
PHST- 1992/03/01 00:00 [entrez]
PHST- 1992/09/01 00:00 [pmc-release]
AID - 10.1073/pnas.89.5.1973 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1992 Mar 1;89(5):1973-7. doi: 10.1073/pnas.89.5.1973.