PMID- 1543541
OWN - NLM
STAT- MEDLINE
DCOM- 19920416
LR  - 20191021
IS  - 0899-1987 (Print)
IS  - 0899-1987 (Linking)
VI  - 5
IP  - 1
DP  - 1992
TI  - Alterations in mRNA levels for growth-related genes after transplantation into 
      castrated hosts in oncogene-induced clonal mouse prostate carcinoma.
PG  - 52-61
AB  - A clonal mouse prostate carcinoma was established by the introduction of the ras 
      and myc oncogenes via the recombinant retrovirus Zipras/myc 9 using a mouse 
      prostate reconstitution model system. A single-cell suspension derived from an 
      early passage ras+myc-induced carcinoma was inoculated into the flanks of intact 
      or castrated adult male C57BL/6 mice, and tumors were harvested 3 wk 
      postinoculation for northern and Southern blotting. Tumor volume analysis showed 
      that this carcinoma was not dependent on testicular androgens for growth. 
      Southern blot analysis of virus-cell DNA junction fragments revealed that tumor 
      cell populations recovered from both intact and castrated mice were progeny of 
      the same virus-infected cell. Northern blotting showed that mRNA levels for the 
      four growth-related genes transforming growth factor-beta 1 (TGF-beta 1), 
      transforming growth factor-beta 3 (TGF-beta 3), tissue-type plasminogen activator 
      (tPA), and c-myc were significantly elevated in clonal mouse prostate carcinomas 
      grown in castrated hosts. In contrast, androgen receptor mRNA levels were 
      significantly reduced under the same conditions. The response of TGF-beta 1, tPA, 
      and c-myc mRNA levels in the carcinomas grown in castrated hosts was similar to 
      that shown previously in normal rat ventral prostate. However, unlike normal rat 
      ventral prostate after castration, increased numbers of apoptotic cells were not 
      seen in the castrated group relative to the intact group at the time of analysis, 
      indicating that the altered gene expression was not associated with cell death. 
      In addition, testosterone-repressed prostate mRNA number 2 levels, shown 
      previously to be elevated after castration in normal rat ventral prostate, were 
      not increased in the androgen-deprived clonal mouse prostate carcinomas. 
      Therefore, this early passage clonal ras+myc-induced prostate carcinoma 
      demonstrates unique patterns of expression for a set of growth-related genes in 
      an androgen-deprived environment.
FAU - Egawa, S
AU  - Egawa S
AD  - Scott Department of Urology, Baylor College of Medicine, Houston, TX 77030.
FAU - Kadmon, D
AU  - Kadmon D
FAU - Miller, G J
AU  - Miller GJ
FAU - Scardino, P T
AU  - Scardino PT
FAU - Thompson, T C
AU  - Thompson TC
LA  - eng
GR  - CA-50588/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Carcinog
JT  - Molecular carcinogenesis
JID - 8811105
RN  - 0 (Androgens)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Neoplasm)
RN  - 0 (Receptors, Androgen)
RN  - 0 (Transcription Factors)
RN  - 0 (Transforming Growth Factor beta)
RN  - EC 3.4.21.68 (Tissue Plasminogen Activator)
RN  - EC 3.4.21.73 (Urokinase-Type Plasminogen Activator)
SB  - IM
MH  - Androgens/physiology
MH  - Animals
MH  - Clone Cells
MH  - Gene Expression Regulation, Neoplastic
MH  - Genes, fos
MH  - Genes, jun
MH  - Genes, myc
MH  - Genes, ras
MH  - Male
MH  - Mice
MH  - Oncogenes
MH  - Orchiectomy
MH  - Prostatic Neoplasms/*genetics/pathology
MH  - Proto-Oncogenes
MH  - RNA, Messenger/genetics
MH  - RNA, Neoplasm/genetics
MH  - Receptors, Androgen/genetics
MH  - Tissue Plasminogen Activator/genetics
MH  - Transcription Factors/genetics
MH  - Transforming Growth Factor beta/genetics
MH  - Tumor Cells, Cultured
MH  - Urokinase-Type Plasminogen Activator/genetics
EDAT- 1992/01/01 00:00
MHDA- 1992/01/01 00:01
CRDT- 1992/01/01 00:00
PHST- 1992/01/01 00:00 [pubmed]
PHST- 1992/01/01 00:01 [medline]
PHST- 1992/01/01 00:00 [entrez]
AID - 10.1002/mc.2940050110 [doi]
PST - ppublish
SO  - Mol Carcinog. 1992;5(1):52-61. doi: 10.1002/mc.2940050110.