PMID- 1544608
OWN - NLM
STAT- MEDLINE
DCOM- 19920415
LR  - 20190827
IS  - 0278-6915 (Print)
IS  - 0278-6915 (Linking)
VI  - 30
IP  - 1
DP  - 1992 Jan
TI  - Comparison of the local lymph node assay with the guinea-pig maximization test 
      for the detection of a range of contact allergens.
PG  - 65-9
AB  - The guinea-pig maximization test (GMPT) has been in use as a method for the 
      prediction of skin sensitization potential for over 20 years, and is widely 
      accepted by regulatory authorities because of its reliable detection of a wide 
      variety of potential human contact allergens. Nevertheless, the method has some 
      limitations and drawbacks, including the use of an adjuvant, the injection of the 
      test substance at induction thus bypassing the normal skin barrier and metabolic 
      function, a subjective endpoint, interference by irritant and/or coloured 
      chemicals, and a relatively long and complex protocol. To address these points, 
      an alternative technique, the local lymph node assay (LLNA), has been proposed 
      and has become the focus of much attention. Recent data from interlaboratory 
      trials have shown a good level of agreement between test facilities and with 
      existing guinea-pig data. The present work investigated the correlation between 
      LLNA results and those derived from the GPMT for 40 chemicals covering a range of 
      chemical types and levels of skin sensitization potential. The LLNA assay was 
      capable of detecting chemicals that exhibit a strong sensitization potential in 
      the GPMT. For chemicals classified as moderate sensitizers in the GPMT, the LLNA 
      was usually positive or provided an indication of sensitizing activity (that was 
      not sufficient to satisfy the current criteria for regarding the result as 
      positive). Weaker sensitizers in the GPMT were usually not detected by the LLNA. 
      With the single exception of copper chloride, non-sensitizers were not positive 
      in the LLNA. The results support the view that the LLNA can provide a rapid and 
      objective screening test for strong sensitizers.
FAU - Basketter, D A
AU  - Basketter DA
AD  - Unilever Environmental Safety Laboratory, Sharnbrook, Bedford, UK.
FAU - Scholes, E W
AU  - Scholes EW
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Food Chem Toxicol
JT  - Food and chemical toxicology : an international journal published for the British 
      Industrial Biological Research Association
JID - 8207483
RN  - 0 (Allergens)
SB  - IM
MH  - Allergens/classification/*toxicity
MH  - Animals
MH  - Guinea Pigs
MH  - Injections, Intradermal
MH  - Lymph Nodes/*drug effects
EDAT- 1992/01/01 00:00
MHDA- 1992/01/01 00:01
CRDT- 1992/01/01 00:00
PHST- 1992/01/01 00:00 [pubmed]
PHST- 1992/01/01 00:01 [medline]
PHST- 1992/01/01 00:00 [entrez]
AID - 0278-6915(92)90138-B [pii]
AID - 10.1016/0278-6915(92)90138-b [doi]
PST - ppublish
SO  - Food Chem Toxicol. 1992 Jan;30(1):65-9. doi: 10.1016/0278-6915(92)90138-b.