PMID- 15447840
OWN - NLM
STAT- MEDLINE
DCOM- 20070504
LR  - 20171213
IS  - 1000-2588 (Print)
IS  - 1000-2588 (Linking)
VI  - 24
IP  - 9
DP  - 2004 Sep
TI  - [Expression of ABCA1 in vascular endothelial cells and its significance in the 
      pathogenesis of atherosclerosis].
PG  - 980-3
AB  - OBJECTIVE: To investigate the changes in the expressions of ATP-binding cassette 
      transporter A1 (ABCA1), intercellular cell adhesion molecule type 1 (ICAM-1) and 
      monocyte chemoattractant protein-1 (MCP-1) at mRNA and protein levels after the 
      treatment of the vascular endothelial ECV304 cells with oxidized low-density 
      lipoprotein (Ox-LDL) and 8-Br-cAMP, thereby to explore possible mechanisms by 
      which ABCA1 affects the formation of atherosclerosis (AS). METHODS: Resuscitated 
      and cultured ECV304 cells were incubated in serum-free medium for 12 h to induce 
      the quiescence phase of growth, which were subsequently treated with Ox-LDL (30 
      ng/ml) and 8-Br-cAMP (0.5 mmol/L) respectively for 3, 6, 12, and 24 h. After the 
      cells were harvested at the specified time points, the mRNA and protein levels of 
      ABCA1, ICAM-1 and MCP-1 were detected by RT-PCR and Western blotting, 
      respectively. RESULTS: The mRNA and protein expression levels of ABCA1, ICAM-1 
      and MCP-1 all increased after treatments with Ox-LDL and 8-Br-cAMP. The highest 
      expression of ICAM-1 occurred in cells with a 12-hour treatment, and those of 
      ABCA1 and MCP-1 occurred following 6-hour incubation with Ox-LDL. The expression 
      peaks of ABCA1, ICAM-1 and MCP-1 all took place after 6-hour incubation with 
      8-Br-cAMP. CONCLUSIONS: The mRNA and protein expressions of ICAM-1 and MCP-1 in 
      vascular endothelial ECV304 cells increase in response to Ox-LDL treatment, in 
      the event of which ABCA1 is also up-regulated to offer protective effects against 
      AS. cAMP not only enhances the expression of ABCA1 but also those of ICAM-1 and 
      MCP-1.
FAU - Li, Jian-hua
AU  - Li JH
AD  - Department of Cardiology, Nanfang Hospital, First Military Medical University, 
      Guangzhou 510515, China. lijhlijh@126.com
FAU - Guo, Zhi-gang
AU  - Guo ZG
FAU - Wu, Ping-sheng
AU  - Wu PS
FAU - Yang, Yong-hong
AU  - Yang YH
FAU - Lai, Wen-yan
AU  - Lai WY
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Di Yi Jun Yi Da Xue Xue Bao
JT  - Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA
JID - 9426110
RN  - 0 (ABCA1 protein, human)
RN  - 0 (ATP Binding Cassette Transporter 1)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (oxidized low density lipoprotein)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 23583-48-4 (8-Bromo Cyclic Adenosine Monophosphate)
SB  - IM
MH  - 8-Bromo Cyclic Adenosine Monophosphate/pharmacology
MH  - ATP Binding Cassette Transporter 1
MH  - ATP-Binding Cassette Transporters/*biosynthesis/genetics
MH  - Atherosclerosis/etiology/*metabolism
MH  - Cells, Cultured
MH  - Chemokine CCL2/biosynthesis/genetics
MH  - Endothelium, Vascular/cytology/*metabolism
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/biosynthesis/genetics
MH  - Lipoproteins, LDL/pharmacology
EDAT- 2004/09/28 05:00
MHDA- 2007/05/05 09:00
CRDT- 2004/09/28 05:00
PHST- 2004/09/28 05:00 [pubmed]
PHST- 2007/05/05 09:00 [medline]
PHST- 2004/09/28 05:00 [entrez]
PST - ppublish
SO  - Di Yi Jun Yi Da Xue Xue Bao. 2004 Sep;24(9):980-3.