PMID- 15447989
OWN - NLM
STAT- MEDLINE
DCOM- 20050111
LR  - 20140728
IS  - 1078-0432 (Print)
IS  - 1078-0432 (Linking)
VI  - 10
IP  - 18 Pt 1
DP  - 2004 Sep 15
TI  - Identification of a novel human cancer/testis antigen, KM-HN-1, recognized by 
      cellular and humoral immune responses.
PG  - 6047-57
AB  - PURPOSE: We used serologic screening of a cDNA expression library of human testis 
      to identify novel cancer/testis antigens that elicit both humoral and cellular 
      immune responses in cancer patients. EXPERIMENTAL DESIGN AND RESULTS: We 
      identified a novel gene designated KM-HN-1 the expression of which is 
      testis-specific among normal tissues; it contains coiled coil domains and a 
      leucine zipper motif and encodes a putative protein consisting of 833 amino 
      acids. KM-HN-1 expression was observed in various cancer tissues and cancer cell 
      lines at both mRNA and protein levels. Immunofluorescence staining of an 
      esophageal cancer cell line revealed that KM-HN-1 protein was present exclusively 
      in the nucleus during mitosis. Recombinant KM-HN-1 protein was produced, and used 
      for ELISA to quantitate levels of IgG antibody specific to KM-HN-1. Higher levels 
      of IgG antibodies specific to KM-HN-1 were detected in many types and numbers of 
      cancer patients but not in healthy donors. The CTL lines specific to KM-HN-1, 
      generated from HLA-A*2402-positive healthy donors and cancer patients, killed 
      human leukocyte antigen (HLA)-A24-positive cancer cells expressing KM-HN-1 but 
      not cell lines that did not express either KM-HN-1 or HLA-A24. CONCLUSIONS: We 
      identified a novel cancer/testis antigen, KM-HN-1, which elicited humoral immune 
      responses in patients with various types of cancer. Furthermore, KM-HN-1-specific 
      CTLs could be generated from both healthy donors and cancer patients, which 
      indicated that KM-HN-1 can be a candidate for an ideal target for cancer 
      immunotherapy.
FAU - Monji, Mikio
AU  - Monji M
AD  - Department of Immunogenetics, , Graduate School of Medical Sciences, Kumamoto 
      University, Kumamoto, Japan.
FAU - Nakatsura, Tetsuya
AU  - Nakatsura T
FAU - Senju, Satoru
AU  - Senju S
FAU - Yoshitake, Yoshihiro
AU  - Yoshitake Y
FAU - Sawatsubashi, Motohiro
AU  - Sawatsubashi M
FAU - Shinohara, Masanori
AU  - Shinohara M
FAU - Kageshita, Toshiro
AU  - Kageshita T
FAU - Ono, Tomomichi
AU  - Ono T
FAU - Inokuchi, Akira
AU  - Inokuchi A
FAU - Nishimura, Yasuharu
AU  - Nishimura Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Clin Cancer Res
JT  - Clinical cancer research : an official journal of the American Association for 
      Cancer Research
JID - 9502500
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (CCDC110 protein, human)
RN  - 0 (DNA, Complementary)
RN  - 0 (Epitopes)
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-A24 Antigen)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Peptides)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 2.5.1.18 (Glutathione Transferase)
RN  - GMW67QNF9C (Leucine)
SB  - IM
MH  - Amino Acid Motifs
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, Neoplasm/*biosynthesis/*chemistry
MH  - Blotting, Northern
MH  - COS Cells
MH  - Cell Line
MH  - Cell Line, Tumor
MH  - Cell Nucleus/metabolism
MH  - Cloning, Molecular
MH  - DNA, Complementary/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epitopes/chemistry
MH  - Fluorescent Antibody Technique, Indirect
MH  - Gene Library
MH  - Genetic Predisposition to Disease
MH  - Glutathione Transferase/metabolism
MH  - HLA-A Antigens/chemistry
MH  - HLA-A24 Antigen
MH  - Humans
MH  - Immune System
MH  - Immunoglobulin G/chemistry
MH  - Immunohistochemistry
MH  - Immunotherapy/*methods
MH  - Interferon-gamma/metabolism
MH  - Leucine/chemistry
MH  - Male
MH  - Microscopy, Fluorescence
MH  - Molecular Sequence Data
MH  - Nuclear Proteins
MH  - Peptides/chemistry
MH  - Protein Structure, Tertiary
MH  - RNA, Messenger/metabolism
MH  - Recombinant Proteins/chemistry
MH  - T-Lymphocytes, Cytotoxic/metabolism
MH  - Testis/*metabolism
MH  - Tissue Distribution
EDAT- 2004/09/28 05:00
MHDA- 2005/01/12 09:00
CRDT- 2004/09/28 05:00
PHST- 2004/09/28 05:00 [pubmed]
PHST- 2005/01/12 09:00 [medline]
PHST- 2004/09/28 05:00 [entrez]
AID - 10/18/6047 [pii]
AID - 10.1158/1078-0432.CCR-04-0475 [doi]
PST - ppublish
SO  - Clin Cancer Res. 2004 Sep 15;10(18 Pt 1):6047-57. doi: 
      10.1158/1078-0432.CCR-04-0475.