PMID- 1544848
OWN - NLM
STAT- MEDLINE
DCOM- 19920414
LR  - 20211119
IS  - 0360-3016 (Print)
IS  - 0360-3016 (Linking)
VI  - 22
IP  - 4
DP  - 1992
TI  - Extracellular: intracellular and subcellular concentration gradients of thiols.
PG  - 751-4
AB  - Chinese hamster V79 cells in Eagle's minimum essential medium in vitro at room 
      temperature were incubated with the aminothiol, WR-1065, or glutathione (GSH) at 
      extracellular concentrations of approximately 1 mmol dm-3. Average intracellular 
      concentrations of GSH, cysteine, and WR-1065 were measured by high performance 
      liquid chromatography, and the effective reducing environment near DNA probed by 
      staining the cells with acridine orange (AO) and measuring the delayed 
      fluorescence. Exposure to either thiol resulted in a rapid, 10-fold increase in 
      average intracellular cysteine concentrations (to about 1 mmol dm-3). Adding 
      extracellular GSH after prior depletion of GSH by treatment with L-buthionine 
      sulfoximine (BSO) did not restore intracellular GSH, but intracellular cysteine 
      was elevated 10-fold. These results are ascribed to thiol/disulfide exchange with 
      cystine in the medium. WR-1065 slowly concentrated intracellularly to 
      approximately 160% of the extracellular concentration. Chemical conjugation of 
      GSH in cells decreased the reducing environment near DNA, but BSO treatment 
      altered the uptake of AO. The electrostatic attraction of WR-1065 toward isolated 
      DNA was markedly affected by ionic strength.
FAU - Wardman, P
AU  - Wardman P
AD  - Gray Laboratory, Cancer Research Campaign, Mount Vernon Hospital, Northwood, 
      Middlesex, U.K.
FAU - Dennis, M F
AU  - Dennis MF
FAU - Stratford, M R
AU  - Stratford MR
FAU - White, J
AU  - White J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Radiat Oncol Biol Phys
JT  - International journal of radiation oncology, biology, physics
JID - 7603616
RN  - 0 (Mercaptoethylamines)
RN  - 0 (Radiation-Protective Agents)
RN  - 05P3K9I49L (N-(2-mercaptoethyl)-1,3-diaminopropane)
RN  - GAN16C9B8O (Glutathione)
RN  - K848JZ4886 (Cysteine)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Chromatography, High Pressure Liquid
MH  - Cricetinae
MH  - Cysteine/analysis
MH  - Extracellular Space/metabolism
MH  - Glutathione/*pharmacokinetics
MH  - In Vitro Techniques
MH  - Intracellular Fluid/metabolism
MH  - Mercaptoethylamines/*pharmacokinetics
MH  - Radiation-Protective Agents/*pharmacokinetics
MH  - Subcellular Fractions/metabolism
EDAT- 1992/01/01 00:00
MHDA- 1992/01/01 00:01
CRDT- 1992/01/01 00:00
PHST- 1992/01/01 00:00 [pubmed]
PHST- 1992/01/01 00:01 [medline]
PHST- 1992/01/01 00:00 [entrez]
AID - 0360-3016(92)90517-L [pii]
AID - 10.1016/0360-3016(92)90517-l [doi]
PST - ppublish
SO  - Int J Radiat Oncol Biol Phys. 1992;22(4):751-4. doi: 
      10.1016/0360-3016(92)90517-l.