PMID- 15448963 OWN - NLM STAT- MEDLINE DCOM- 20050104 LR - 20160512 IS - 1618-2642 (Print) IS - 1618-2642 (Linking) VI - 380 IP - 4 DP - 2004 Oct TI - Simultaneous determination of L-arginine and its mono- and dimethylated metabolites in human plasma by high-performance liquid chromatography-mass spectrometry. PG - 643-9 AB - A simple, fast, sensitive, and reproducible isocratic liquid chromatography-mass spectrometry (LC-MS) method coupled with an atmospheric pressure chemical ionization (APCI) interface for simultaneous separation and determination of L-arginine (ARG) and its methylated metabolites, N-monomethyl- L-arginine (MMA), NG, NG-dimethylarginine (asymmetric dimethyl arginine, ADMA), and NG, N'G-dimethylarginine (symmetric dimethyl arginine, SDMA), in human plasma is presented. Sample pretreatment is not required other than deproteinization with 5-sulfosalicylic acid (5-SSA). Satisfactory chromatographic separation was achieved on a 2.0x150-mm Shimadzu VP-ODS column by using a mobile phase consisting of water/acetonitrile (90/10, v/v) containing 0.5% trifluoroacetic acid (TFA). Positive selective ion monitoring (SIM) mode was chosen for quantification of each analyte. The positively protonated molecular ions [M+H]+ of ARG, MMA, ADMA, and SDMA were monitored at m/z 175, 189, 203, and 203, respectively. L-Homoarginine was used as the internal standard (IS) for the assay. The limits of quantification (LOQs) were found to be 1.0 micromol L(-1) for ARG, and 0.2 micromol L(-1) for MMA, ADMA, and SDMA. The inter-assay precision and accuracy were in the range of 1.8-4.9% and -3.0-5.0%, respectively. The intra-assay precision and accuracy were in the order of 1.7-4.6 and -2.6-4.0%, respectively. The recoveries were between 90.0 and 106.6%. The levels of ARG, MMA, ADMA, and SDMA in human plasma were also determined using the developed method. FAU - Huang, Lan-Fang AU - Huang LF AD - College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China. lf18huang@yahoo.com.cn FAU - Guo, Fang-Qiu AU - Guo FQ FAU - Liang, Yi-Zeng AU - Liang YZ FAU - Li, Bo-Yang AU - Li BY FAU - Cheng, Ben-Mei AU - Cheng BM LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20040924 PL - Germany TA - Anal Bioanal Chem JT - Analytical and bioanalytical chemistry JID - 101134327 RN - 94ZLA3W45F (Arginine) SB - IM MH - Arginine/*blood/chemistry/*metabolism MH - Chromatography, High Pressure Liquid/*methods MH - Humans MH - Mass Spectrometry/*methods MH - Methylation MH - Molecular Structure EDAT- 2004/09/28 05:00 MHDA- 2005/01/05 09:00 CRDT- 2004/09/28 05:00 PHST- 2004/05/17 00:00 [received] PHST- 2004/07/04 00:00 [revised] PHST- 2004/07/06 00:00 [accepted] PHST- 2004/09/28 05:00 [pubmed] PHST- 2005/01/05 09:00 [medline] PHST- 2004/09/28 05:00 [entrez] AID - 10.1007/s00216-004-2759-y [doi] PST - ppublish SO - Anal Bioanal Chem. 2004 Oct;380(4):643-9. doi: 10.1007/s00216-004-2759-y. Epub 2004 Sep 24.