PMID- 15450809
OWN - NLM
STAT- MEDLINE
DCOM- 20050815
LR  - 20061115
IS  - 0003-2697 (Print)
IS  - 0003-2697 (Linking)
VI  - 333
IP  - 2
DP  - 2004 Oct 15
TI  - Application of solid-phase microextraction combined with derivatization to the 
      determination of amphetamines by liquid chromatography.
PG  - 328-35
AB  - This work evaluates the utility of solid-phase microextraction (SPME) in the 
      analysis of amphetamines by liquid chromatography (LC) after chemical 
      derivatization of the analytes. Two approaches have been tested and compared, 
      SPME followed by on-fiber derivatization of the extracted amphetamines, and 
      solution derivatization followed by SPME of the derivatives formed. Both methods 
      have been applied to measure amphetamine (AP), methamphetamine (MA), and 
      3,4-methylenedioxymethamphetamine (MDMA), using the fluorogenic reagent 
      9-fluorenylmethyl chloroformate (FMOC) and carbowax-templated resin 
      (CW-TR)-coated fibers. Data on the application of the proposed methods for the 
      analysis of different kind of samples are presented. When analyzing aqueous 
      solutions of the analytes, both approaches gave similar analytical performance, 
      but the sensitivity attainable with the solution derivatization/SPME method was 
      better. The efficiencies observed when processing spiked urine samples by the 
      SPME/on-fiber derivatization approach were very low. This was because the 
      extraction of matrix components into the fiber coating prevented the extraction 
      of the reagent. In contrast, the efficiencies obtained for spiked urine samples 
      by the solution derivatization/SPME approach were similar to those obtained for 
      aqueous samples. Therefore, the later method would be the method of choice for 
      the quantification of amphetamines in urine.
FAU - Chafer-Pericas, C
AU  - Chafer-Pericas C
AD  - Department of Analytical Chemistry, University of Valencia, Dr. Moliner 50, 46100 
      Burjassot, Valencia, Spain.
FAU - Campins-Falco, P
AU  - Campins-Falco P
FAU - Herraez-Hernandez, R
AU  - Herraez-Hernandez R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Anal Biochem
JT  - Analytical biochemistry
JID - 0370535
RN  - 0 (Amphetamines)
SB  - IM
MH  - Amphetamines/*analysis/*chemistry/isolation & purification/urine
MH  - Chromatography, High Pressure Liquid
MH  - Microchemistry/*methods
MH  - Reference Values
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
EDAT- 2004/09/29 05:00
MHDA- 2005/08/16 09:00
CRDT- 2004/09/29 05:00
PHST- 2004/04/28 00:00 [received]
PHST- 2004/09/29 05:00 [pubmed]
PHST- 2005/08/16 09:00 [medline]
PHST- 2004/09/29 05:00 [entrez]
AID - S0003269704004956 [pii]
AID - 10.1016/j.ab.2004.05.056 [doi]
PST - ppublish
SO  - Anal Biochem. 2004 Oct 15;333(2):328-35. doi: 10.1016/j.ab.2004.05.056.