PMID- 15450858
OWN - NLM
STAT- MEDLINE
DCOM- 20050315
LR  - 20151119
IS  - 1532-0456 (Print)
IS  - 1532-0456 (Linking)
VI  - 138
IP  - 2
DP  - 2004 Jun
TI  - Effects of superoxide dismutase and polyclonal tumor necrosis factor-alpha 
      antibodies on chloroacetate-induced cellular death and superoxide anion 
      production by J774.A1 macrophages.
PG  - 113-20
AB  - Dichloroacetate (DCA) and trichloroacetate (TCA) are by-products that are formed 
      during the process of water chlorination and have been previously shown to induce 
      superoxide anion (SA) production and cellular death when added to J774.A1 
      macrophage cultures. In this study, the effects of superoxide dismutase (SOD) and 
      polyclonal tumor necrosis factor-alpha (TNF-alpha) antibodies on DCA- and 
      TCA-induced SA production and cellular death have been tested on the J774.A1 
      macrophage cultures. TCA and DCA were added to different cultures either alone, 
      each at a concentration of 16 mM, or in combination with SOD (2-12 units/ml), or 
      with TNF-alpha antibodies (10 and 25 units/ml). Cells were incubated for 48 h, 
      after which cellular death/viability, lactate dehydrognase (LDH) leakage by the 
      cells, and SA production by the cells were determined. While TCA and DCA caused 
      significant cellular toxicity, indicated by reduction in cellular viability and 
      increases in LDH leakage and SA production, SOD addition resulted in significant 
      reduction of the effects induced by the compounds. On the other hand, addition of 
      TNF-alpha antibodies to the DCA- and TCA-treated cultures resulted in significant 
      reduction of DCA- but not TCA-induced cellular death and SA production by the 
      cells. Although these results suggest a significant role for SA in DCA- and 
      TCA-induced cellular death, they may also suggest two different mechanisms for 
      the chloroacetate-induced SA production by the cells.
FAU - Hassoun, Ezdihar A
AU  - Hassoun EA
AD  - Department of Pharmacology, College of Pharmacy, The University of Toledo, 2801 
      W. Bancroft Street, OH 43606, USA. ehassou@utnet.utoledo.edu
FAU - Kini, Vidisha
AU  - Kini V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Comp Biochem Physiol C Toxicol Pharmacol
JT  - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
JID - 100959500
RN  - 0 (Acetates)
RN  - 0 (Antibodies)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 11062-77-4 (Superoxides)
RN  - 5GD84Y125G (chloroacetic acid)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
SB  - IM
MH  - Acetates/*toxicity
MH  - Animals
MH  - Antibodies/pharmacology
MH  - Cell Death/drug effects/physiology
MH  - Cell Line
MH  - Cell Survival/drug effects/physiology
MH  - Macrophages/*drug effects/metabolism
MH  - Mice
MH  - Superoxide Dismutase/*pharmacology
MH  - Superoxides/*metabolism
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 2004/09/29 05:00
MHDA- 2005/03/16 09:00
CRDT- 2004/09/29 05:00
PHST- 2004/01/28 00:00 [received]
PHST- 2004/05/07 00:00 [revised]
PHST- 2004/05/08 00:00 [accepted]
PHST- 2004/09/29 05:00 [pubmed]
PHST- 2005/03/16 09:00 [medline]
PHST- 2004/09/29 05:00 [entrez]
AID - S1532045604001000 [pii]
AID - 10.1016/j.cca.2004.05.008 [doi]
PST - ppublish
SO  - Comp Biochem Physiol C Toxicol Pharmacol. 2004 Jun;138(2):113-20. doi: 
      10.1016/j.cca.2004.05.008.