PMID- 15450965
OWN - NLM
STAT- MEDLINE
DCOM- 20041221
LR  - 20061115
IS  - 1050-4648 (Print)
IS  - 1050-4648 (Linking)
VI  - 18
IP  - 1
DP  - 2005 Jan
TI  - Protection against Atlantic halibut nodavirus in turbot is induced by recombinant 
      capsid protein vaccination but not following DNA vaccination.
PG  - 13-29
AB  - Fish nodaviruses (betanodaviruses) are small, non-enveloped icosahedral 
      single-stranded positive-sense RNA viruses that can cause viral encephalopathy 
      and retinopathy (VER) in a number of cultured marine teleost species, including 
      Atlantic halibut (Hippoglossus hippoglossus). A recombinant protein vaccine and a 
      DNA vaccine were produced, based on the same capsid-encoding region of the 
      Atlantic halibut nodavirus (AHNV) genome, and tested for protection in juvenile 
      turbot (Scophthalmus maximus). Vaccine efficacy was demonstrated in the fish 
      vaccinated with recombinant capsid protein but not in the DNA-vaccinated fish, 
      despite the fact that in vivo expression of the DNA vaccine-encoded antigen was 
      confirmed by RNA in situ hybridisation and immunohistochemistry. Combined DNA and 
      recombinant vaccine administration did not improve the effect of the latter. 
      Surprisingly, fish vaccinated with 50 microg recombinant protein demonstrated a 
      threefold lower survival rate than the two groups that received 10 microg 
      recombinant protein. Neither the recombinant protein vaccine nor the DNA vaccine 
      induced anti-viral antibodies 9 weeks after immunisation, while antibodies 
      reactive with the recombinant protein were detectable mainly in fish vaccinated 
      with 50 microg recombinant protein. The study also demonstrates evidence of viral 
      replication inside the myocytes of intramuscularly challenged fish.
FAU - Sommerset, Ingunn
AU  - Sommerset I
AD  - Institute of Marine Research, PO Box 1870 Nordnes, N-5817 Bergen, Norway. 
      ingunn.sommerset@intervet.com
FAU - Skern, Rasmus
AU  - Skern R
FAU - Biering, Eirik
AU  - Biering E
FAU - Bleie, Hogne
AU  - Bleie H
FAU - Fiksdal, Ingrid Uglenes
AU  - Fiksdal IU
FAU - Grove, Soren
AU  - Grove S
FAU - Nerland, Audun Helge
AU  - Nerland AH
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Capsid Proteins)
RN  - 0 (Vaccines, DNA)
RN  - 0 (Vaccines, Synthetic)
SB  - IM
MH  - Animals
MH  - Aquaculture
MH  - Capsid Proteins/*immunology
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - Fish Diseases/*immunology/*prevention & control/virology
MH  - Flatfishes
MH  - Immunohistochemistry/veterinary
MH  - In Situ Hybridization/veterinary
MH  - Neutralization Tests/veterinary
MH  - Nodaviridae/*immunology
MH  - RNA Virus Infections/immunology/prevention & control/*veterinary
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Time Factors
MH  - Vaccination/*veterinary
MH  - Vaccines, DNA/immunology/metabolism
MH  - Vaccines, Synthetic/immunology
EDAT- 2004/09/29 05:00
MHDA- 2004/12/22 09:00
CRDT- 2004/09/29 05:00
PHST- 2004/03/29 00:00 [accepted]
PHST- 2004/09/29 05:00 [pubmed]
PHST- 2004/12/22 09:00 [medline]
PHST- 2004/09/29 05:00 [entrez]
AID - S1050464804000191 [pii]
AID - 10.1016/j.fsi.2004.03.006 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2005 Jan;18(1):13-29. doi: 10.1016/j.fsi.2004.03.006.