PMID- 15451381
OWN - NLM
STAT- MEDLINE
DCOM- 20050106
LR  - 20131121
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 1024
IP  - 1-2
DP  - 2004 Oct 22
TI  - The effect of escitalopram, desipramine, electroconvulsive seizures and lithium 
      on brain-derived neurotrophic factor mRNA and protein expression in the rat brain 
      and the correlation to 5-HT and 5-HIAA levels.
PG  - 183-92
AB  - The reported increase in brain-derived neurotrophic factor (BDNF) mRNA expression 
      after antidepressant treatment is a cornerstone of the BDNF hypothesis of 
      antidepressant action. However, if this increase becomes manifest on the BDNF 
      protein level is unknown. In the present study we performed parallel measurements 
      of BDNF mRNA and protein expression in the frontal cortex and hippocampus of the 
      rat after chronic treatment with electroconvulsive seizures (ECS), lithium, 
      desipramine or escitalopram. ECS increased BDNF mRNA and protein in the 
      hippocampus and BDNF protein in the frontal cortex. Desipramine moderately 
      increased BDNF mRNA expression in the dentate gyrus but did not change BDNF 
      protein in neither region. Escitalopram did not affect BDNF mRNA expression, but 
      decreased BDNF protein in the frontal cortex and the hippocampus. Lithium 
      increased BDNF protein levels in the hippocampus and frontal cortex, but overall 
      decreased BDNF mRNA expression. Thus, here we report a striking 
      non-correspondence between changes in BDNF mRNA and protein expression induced by 
      the antidepressant treatments and lithium. Further, increased expression of BDNF 
      mRNA or protein was not a common action of the treatments. We also investigated 
      if treatment-induced modulations of the tissue contents of 5-hydroxytryptamine 
      (5-HT) and its metabolite, 5-hydroxy-indoleacetic acid (5-HIAA), were related to 
      changes in BDNF mRNA or protein expression. No correlation was found. However, 
      all treatments increased 5-HT levels in the hippocampus.
FAU - Jacobsen, Jacob Pade Ramsoe
AU  - Jacobsen JP
AD  - Department of Neurochemistry, H. Lundbeck A/S, Ottiliavej 9, DK-2500 Valby, 
      Denmark. jprj@lundbeck.com
FAU - Mork, Arne
AU  - Mork A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0DHU5B8D6V (Citalopram)
RN  - 333DO1RDJY (Serotonin)
RN  - 54-16-0 (Hydroxyindoleacetic Acid)
RN  - 9FN79X2M3F (Lithium)
RN  - TG537D343B (Desipramine)
SB  - IM
MH  - Animals
MH  - Brain/drug effects/metabolism
MH  - Brain-Derived Neurotrophic Factor/*biosynthesis/genetics
MH  - Citalopram/*pharmacology
MH  - Desipramine/*pharmacology
MH  - Electroshock/methods
MH  - Gene Expression Regulation/drug effects/physiology
MH  - Hydroxyindoleacetic Acid/metabolism
MH  - Lithium/*pharmacology
MH  - Male
MH  - Proteins/genetics/metabolism
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Rats
MH  - Rats, Wistar
MH  - Seizures/genetics/*metabolism
MH  - Serotonin/*biosynthesis/genetics
EDAT- 2004/09/29 05:00
MHDA- 2005/01/07 09:00
CRDT- 2004/09/29 05:00
PHST- 2004/07/31 00:00 [accepted]
PHST- 2004/09/29 05:00 [pubmed]
PHST- 2005/01/07 09:00 [medline]
PHST- 2004/09/29 05:00 [entrez]
AID - S0006-8993(04)01254-5 [pii]
AID - 10.1016/j.brainres.2004.07.065 [doi]
PST - ppublish
SO  - Brain Res. 2004 Oct 22;1024(1-2):183-92. doi: 10.1016/j.brainres.2004.07.065.