PMID- 15454691
OWN - NLM
STAT- MEDLINE
DCOM- 20050324
LR  - 20181113
IS  - 0282-0080 (Print)
IS  - 0282-0080 (Linking)
VI  - 20
IP  - 9
DP  - 2004
TI  - Identification of 9-O-acetylated sialoglycans on peripheral blood mononuclear 
      cells in Indian Visceral Leishmaniasis.
PG  - 531-6
AB  - Although the existence of O -acetylated sialic acids is well known, it is only in 
      recent years that steady refinement of analytical techniques have enabled 
      detailed mapping of their structural diversity [1]. Fluorimetric analysis of 
      peripheral blood mononuclear cells (PBMC) of patients with Visceral Leishmaniasis 
      (VL) showed six fold increase in the percentage of surface 9-O-acetylated 
      sialoglycoconjugates (9-O-AcSGs) as compared to normal human donors. Using 
      Achatinin-H, a 9-O-acetyl sialic acid- binding lectin, an enhanced presence of 
      9-O-AcSGs in an alpha2 --> 6 linkage was demonstrated by flow cytometry; 
      abolition of its binding by pretreatment with a recombinant 9-O-acetylesterase 
      corroborated the presence of this glycotope. Western blotting of PBMC from VL 
      patients indicated the presence of five O-acetylated sialoglycans corresponding 
      to 144, 65, 56, 36 and 19 kDa as compared to 144 and 36 kDa in normal 
      individuals. Taken together our data indicates that during active disease, there 
      is an overexpression of 9AcSGs on the surface of PBMC of VL patients, thus 
      opening up new research avenues wherein the expression of this biomarker could be 
      exploited to monitor the clinical status of VL patients.
FAU - Bandyopadhyay, Sumi
AU  - Bandyopadhyay S
AD  - Immunobiology Division, Indian Institute of Chemical Biology, 4 Raja S.C. Mullick 
      Road, Kolkata 700 032, India.
FAU - Chatterjee, Mitali
AU  - Chatterjee M
FAU - Sundar, Shyam
AU  - Sundar S
FAU - Mandal, Chitra
AU  - Mandal C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Glycoconj J
JT  - Glycoconjugate journal
JID - 8603310
RN  - 0 (Biomarkers)
RN  - 0 (Lectins)
RN  - 0 (Polysaccharides)
RN  - 0 (Sialic Acids)
RN  - 0 (achatinin(H))
RN  - GZP2782OP0 (N-Acetylneuraminic Acid)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Biomarkers
MH  - Blotting, Western
MH  - Cell Proliferation
MH  - Child
MH  - Child, Preschool
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Flow Cytometry
MH  - Glycosylation
MH  - Humans
MH  - Lectins/chemistry
MH  - Leishmaniasis, Visceral/*blood
MH  - Leukocytes, Mononuclear/*metabolism
MH  - Lymphocytes/metabolism
MH  - Microscopy, Fluorescence
MH  - Middle Aged
MH  - N-Acetylneuraminic Acid/chemistry
MH  - Polysaccharides/*chemistry
MH  - Protein Binding
MH  - Sialic Acids/*chemistry
EDAT- 2004/09/30 05:00
MHDA- 2005/03/25 09:00
CRDT- 2004/09/30 05:00
PHST- 2004/09/30 05:00 [pubmed]
PHST- 2005/03/25 09:00 [medline]
PHST- 2004/09/30 05:00 [entrez]
AID - 5277284 [pii]
AID - 10.1023/B:GLYC.0000043289.86611.44 [doi]
PST - ppublish
SO  - Glycoconj J. 2004;20(9):531-6. doi: 10.1023/B:GLYC.0000043289.86611.44.