PMID- 15454780
OWN - NLM
STAT- MEDLINE
DCOM- 20041202
LR  - 20040929
IS  - 0023-852X (Print)
IS  - 0023-852X (Linking)
VI  - 114
IP  - 10
DP  - 2004 Oct
TI  - mRNA for genes associated with antigen presentation are expressed by human middle 
      meatal epithelial cells in culture.
PG  - 1827-32
AB  - OBJECTIVES/HYPOTHESIS: Although the mechanisms underlying the initiation and 
      maintenance of inflammation in chronic rhinosinusitis are poorly understood, the 
      activation of memory T cells within the nasal mucosa is thought to play an 
      important role. T-cell activation requires specialized antigen processing and 
      presentation of antigen by immunocompetent cells in the context of cell surface 
      immune molecules. The purpose of this study was to investigate the expression of 
      such molecules by human sinonasal epithelial cells grown in culture at the 
      air-liquid interface (ALI). METHODS: Middle meatal epithelium was obtained from 
      six patients undergoing endoscopic sinus surgery. Dissociated epithelial cells 
      were grown to confluence in serum-free, defined medium and transferred to filter 
      inserts for culture at the ALI. Cells were harvested at 2 and 21 days of growth 
      at the ALI and processed for real-time polymerase chain reaction (PCR). The 
      presence and relative abundance of constitutively expressed mRNA for human 
      leukocyte antigen (HLA)-B, HLA-DR, B7 to 1, B7 to 2, B7-H2, B7-H3, and cathepsin 
      D were assessed. RESULTS: After 2 days at the ALI, middle meatal epithelial cells 
      demonstrated expression of genes for each of the antigen processing associated 
      genes tested. The expression of HLA-B and HLA-DR increased significantly with 
      cellular maturation at the ALI. Expression of HLA-DR and B7 to 1 increased with 
      cytokine stimulation. CONCLUSIONS: Primary human epithelial cells obtained from 
      the middle meatus express genes associated with antigen presentation function. 
      The pattern of gene expression is modulated by cytokine stimulation and changes 
      as the cells differentiate at the ALI. These findings suggest that mature middle 
      meatal epithelial cells have the cellular machinery to interact with T cells and 
      therefore may be direct participants in the modulation of T-cell activity in 
      chronic sinusitis.
FAU - Lane, Andrew P
AU  - Lane AP
AD  - Department of Otolaryngology-Head and Neck Surgery, The Johns Hopkins University 
      School of Medicine, 601 N. Caroline Street, Baltimore, MD 21287, USA. 
      alane3@jhmi.edu
FAU - Saatian, Bahman
AU  - Saatian B
FAU - Yu, Xiao-Ying
AU  - Yu XY
FAU - Spannhake, Ernst William
AU  - Spannhake EW
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Laryngoscope
JT  - The Laryngoscope
JID - 8607378
RN  - 0 (Cytokines)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Antigen Presentation/genetics/*immunology
MH  - Cytokines/immunology
MH  - Epithelial Cells/immunology
MH  - Female
MH  - Gene Expression/genetics
MH  - Genes, MHC Class II/genetics/*immunology
MH  - Humans
MH  - Male
MH  - Nasal Mucosa/*immunology
MH  - RNA, Messenger/genetics/*immunology
MH  - Sinusitis/genetics/*immunology
EDAT- 2004/09/30 05:00
MHDA- 2004/12/16 09:00
CRDT- 2004/09/30 05:00
PHST- 2004/09/30 05:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/09/30 05:00 [entrez]
AID - 00005537-200410000-00028 [pii]
AID - 10.1097/00005537-200410000-00028 [doi]
PST - ppublish
SO  - Laryngoscope. 2004 Oct;114(10):1827-32. doi: 10.1097/00005537-200410000-00028.