PMID- 15457367
OWN - NLM
STAT- MEDLINE
DCOM- 20050202
LR  - 20181113
IS  - 0364-2313 (Print)
IS  - 0364-2313 (Linking)
VI  - 28
IP  - 8
DP  - 2004 Aug
TI  - Dependence of positive effects of granulocyte colony-stimulating factor on the 
      antibiotic regimen: evaluation in rats with polymicrobial peritonitis.
PG  - 834-44
AB  - We tested the hypothesis that the ability of granulocyte colony-stimulating 
      factor (G-CSF) to prevent death from fecal peritonitis is influenced by the 
      composition of the antibiotic regimen with which it is administered. We used a 
      rodent model of polymicrobial peritoneal contamination and infection and the 
      concept of clinical modeling randomized trials (CMRTs), which includes the 
      conditions of randomized, clinical trials and complex clinical interventions 
      (e.g., anesthesia, volume substitution, antibiotics, surgery, postoperative 
      analgesia). With the peritonitis model we obtained a mortality dose-response 
      curve that was sensitive to antibiotic prophylaxis. G-CSF was most efficacious 
      when it was administered both prophylactically and after the onset of 
      peritonitis. Cefuroxime/metronidazole, ofloxacin/metronidazole, and 
      amoxicillin/clavulanate improved survival in combination with G-CSF best, whereas 
      cefotaxime or ceftriaxone with and without metronidazole did not. G-CSF 
      administration was associated with improved polymorphonuclear neutrophil 
      phagocytosis and enhanced bacterial clearance. Pro-inflammatory cytokine release 
      (tumor necrosis factor-a, interleukin-6, macrophage inflammatory protein-2) was 
      decreased in plasma and in the peritoneal fluid. Their expression was lowered in 
      various organs on the protein and mRNA level. The results were used to design a 
      clinical trial to test the ability of G-CSF to prevent serious infections in 
      patients with colorectal cancer surgery. In this trial G-CSF application and 
      antibiotic prophylaxis were performed with the most effective scheduling and 
      combinations (cefuroxime/metronidazole and ofloxacin/metronidazole) as defined 
      here.
FAU - Bauhofer, Artur
AU  - Bauhofer A
AD  - Institute of Theoretical Surgery, Philipps-University Marburg, Baldingerstrasse, 
      35043, Marburg, Germany. bauhofer@staff.uni-marburg.de
FAU - Torossian, Alexander
AU  - Torossian A
FAU - Lorenz, Wilfried
AU  - Lorenz W
FAU - Middeke, Martin
AU  - Middeke M
FAU - Plaul, Ulrike
AU  - Plaul U
FAU - Schutz, Philipp
AU  - Schutz P
FAU - Stinner, Benno
AU  - Stinner B
FAU - Hattel, Markus
AU  - Hattel M
FAU - Celik, Ilhan
AU  - Celik I
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20040803
PL  - United States
TA  - World J Surg
JT  - World journal of surgery
JID - 7704052
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
RN  - 143011-72-7 (Granulocyte Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - *Anti-Bacterial Agents
MH  - Antibiotic Prophylaxis
MH  - Bacterial Infections/*immunology/microbiology
MH  - Cytokines/blood
MH  - Dose-Response Relationship, Drug
MH  - Drug Evaluation, Preclinical
MH  - Drug Synergism
MH  - Drug Therapy, Combination/*pharmacology
MH  - Feces/microbiology
MH  - Female
MH  - Granulocyte Colony-Stimulating Factor/*pharmacology
MH  - Humans
MH  - Inflammation Mediators/blood
MH  - Male
MH  - Neutrophils/drug effects/immunology
MH  - Peritonitis/*immunology/microbiology
MH  - Postoperative Complications/*immunology/microbiology
MH  - Rats
EDAT- 2004/10/01 05:00
MHDA- 2005/02/03 09:00
CRDT- 2004/10/01 05:00
PHST- 2004/10/01 05:00 [pubmed]
PHST- 2005/02/03 09:00 [medline]
PHST- 2004/10/01 05:00 [entrez]
AID - 10.1007/s00268-004-7210-1 [doi]
PST - ppublish
SO  - World J Surg. 2004 Aug;28(8):834-44. doi: 10.1007/s00268-004-7210-1. Epub 2004 
      Aug 3.