PMID- 15457925
OWN - NLM
STAT- MEDLINE
DCOM- 20050131
LR  - 20191109
IS  - 1138-7548 (Print)
IS  - 1138-7548 (Linking)
VI  - 60
IP  - 2
DP  - 2004 Jun
TI  - Effect of leptin on renal ischemia-reperfusion damage in rats.
PG  - 79-84
AB  - Tumor necrosis factor-alpha (TNF-alpha) has been established as an important 
      mediator in renal ischemia-reperfusion (I/R) injury. Leptin, a product of the ob 
      gene, has been known to exhibit cytoprotective effects on renal tissue, but its 
      effect on renal tissue TNF-alpha level after renal I/R injury in rats remains 
      unknown. The purpose of the study was to evaluate the effects of leptin on renal 
      tissue TNF-alpha, malondialdehyde (MDA), protein carbonyls (PCs) and total 
      sulfydryl group (SH) levels, and plasma nitrite levels after renal I/R injury in 
      rats. The animals were divided into three groups: control, I/R and I/R+leptin. 
      Rats were subjected to renal ischemia by clamping the left pedicle for 45 min, 
      and then reperfused for 1 h. The I/R+leptin group was pretreated 
      intraperitoneally with leptin (10 microg/kg) 30 min before the induction of 
      ischemia. Our results indicate that MDA, TNF-alpha levels, and PCs were 
      significantly higher in the I/R group than those in the control group (p < 0.05). 
      The administration of leptin decreased these parameters (p < 0.05) significantly. 
      The SH level was observed to significantly decrease after I/R injury when 
      compared to the control group (p < 0.05). Leptin treatment significantly 
      increased tissue SH and plasma nitrite levels when compared to the I/R group (p < 
      0.05). Plasma nitrite levels did not change significantly in I/R when compared to 
      the control. These results suggest that leptin could exert a protective effect on 
      I/R induced renal damage by decreasing TNF-alpha levels and increasing nitrite 
      level.
FAU - Erkasap, S
AU  - Erkasap S
AD  - Department of General Surgery, Osmangazi University, Medical Faculty, Eskisehir, 
      Turkey. serkasap@ogu.edu.tr
FAU - Erkasap, N
AU  - Erkasap N
FAU - Koken, T
AU  - Koken T
FAU - Kahraman, A
AU  - Kahraman A
FAU - Uzuner, K
AU  - Uzuner K
FAU - Yazihan, N
AU  - Yazihan N
FAU - Ates, E
AU  - Ates E
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Spain
TA  - J Physiol Biochem
JT  - Journal of physiology and biochemistry
JID - 9812509
RN  - 0 (Cryoprotective Agents)
RN  - 0 (Leptin)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 4Y8F71G49Q (Malondialdehyde)
SB  - IM
MH  - Animals
MH  - Cryoprotective Agents/*pharmacology
MH  - Disease Models, Animal
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Ischemia/etiology/physiopathology/*therapy
MH  - Kidney Diseases/physiopathology/*therapy
MH  - Leptin/*pharmacology
MH  - Lipid Peroxidation/drug effects
MH  - Malondialdehyde/analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reperfusion Injury/pathology/*prevention & control
MH  - Tumor Necrosis Factor-alpha/analysis
EDAT- 2004/10/02 05:00
MHDA- 2005/02/03 09:00
CRDT- 2004/10/02 05:00
PHST- 2004/10/02 05:00 [pubmed]
PHST- 2005/02/03 09:00 [medline]
PHST- 2004/10/02 05:00 [entrez]
AID - 10.1007/BF03168443 [doi]
PST - ppublish
SO  - J Physiol Biochem. 2004 Jun;60(2):79-84. doi: 10.1007/BF03168443.