PMID- 15458815
OWN - NLM
STAT- MEDLINE
DCOM- 20050202
LR  - 20061115
IS  - 0163-7827 (Print)
IS  - 0163-7827 (Linking)
VI  - 43
IP  - 5
DP  - 2004 Sep
TI  - Matrix-assisted laser desorption and ionization time-of-flight (MALDI-TOF) mass 
      spectrometry in lipid and phospholipid research.
PG  - 449-88
AB  - The interest in the analysis of lipids and phospholipids is continuously 
      increasing due to the importance of these molecules in biochemistry (e.g. in the 
      context of biomembranes and lipid second messengers) as well as in industry. 
      Unfortunately, commonly used methods of lipid analysis are often time-consuming 
      and tedious because they include previous separation and/or derivatization steps. 
      With the development of "soft-ionization techniques" like electrospray ionization 
      (ESI) or matrix-assisted laser desorption and ionization time-of-flight 
      (MALDI-TOF), mass spectrometry became also applicable to lipid analysis. The aim 
      of this review is to summarize so far available experiences in MALDI-TOF mass 
      spectrometric analysis of lipids. It will be shown that MALDI-TOF MS can be 
      applied to all known lipid classes and the characteristics of individual lipids 
      will be discussed. Additionally, some selected applications in medicine and 
      biology, e.g. mixture analysis, cell and tissue analysis and the determination of 
      enzyme activities will be described. Advantages and disadvantages of MALDI-TOF MS 
      in comparison to other established lipid analysis methods will be also discussed.
FAU - Schiller, J
AU  - Schiller J
AD  - Medical Department, Institute of Medical Physics and Biophysics, University of 
      Leipzig, Liebigstrasse 27, D-04103 Leipzig, Germany. schij@medizin.uni-leipzig.de
FAU - Suss, R
AU  - Suss R
FAU - Arnhold, J
AU  - Arnhold J
FAU - Fuchs, B
AU  - Fuchs B
FAU - Lessig, J
AU  - Lessig J
FAU - Muller, M
AU  - Muller M
FAU - Petkovic, M
AU  - Petkovic M
FAU - Spalteholz, H
AU  - Spalteholz H
FAU - Zschornig, O
AU  - Zschornig O
FAU - Arnold, K
AU  - Arnold K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - England
TA  - Prog Lipid Res
JT  - Progress in lipid research
JID - 7900832
RN  - 0 (Lipids)
RN  - 0 (Phospholipids)
RN  - 0 (Plant Oils)
SB  - IM
MH  - Brain Chemistry
MH  - Humans
MH  - Lens, Crystalline/chemistry
MH  - Lipid Peroxidation
MH  - Lipids/*analysis
MH  - Phospholipids/analysis
MH  - Plant Oils/chemistry
MH  - Plants/chemistry
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods
RF  - 162
EDAT- 2004/10/02 05:00
MHDA- 2005/02/03 09:00
CRDT- 2004/10/02 05:00
PHST- 2004/10/02 05:00 [pubmed]
PHST- 2005/02/03 09:00 [medline]
PHST- 2004/10/02 05:00 [entrez]
AID - S0163-7827(04)00026-8 [pii]
AID - 10.1016/j.plipres.2004.08.001 [doi]
PST - ppublish
SO  - Prog Lipid Res. 2004 Sep;43(5):449-88. doi: 10.1016/j.plipres.2004.08.001.