PMID- 15459235
OWN - NLM
STAT- MEDLINE
DCOM- 20050125
LR  - 20171116
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 76
IP  - 6
DP  - 2004 Dec
TI  - Recombinant HLA-G5 and -G6 drive U937 myelomonocytic cell production of 
      TGF-beta1.
PG  - 1220-8
AB  - Throughout human pregnancy, activated maternal macrophages producing 
      anti-inflammatory cytokines comprise a stable cell population in the uterus. This 
      organ is also massively infiltrated with semiallogeneic, placenta-derived, 
      invasive cytotrophoblast cells, which produce membrane and soluble isoforms of 
      human leukocyte antigen (HLA)-G. Here, we investigated the possibility that two 
      soluble isoforms of HLA-G, HLA-G5 and -G6, program macrophage production of 
      cytokines. The model system consisted of human U937 myelomonocytic cells treated 
      with phorbol 12-myristate 13-acetate (PMA) and interferon-gamma (IFN-gamma), 
      which induced differentiation and activation but did not affect their viability 
      or decrease their expression of the two inhibitory immunoglobulin-like transcript 
      (ILT) receptors for HLA-G, ILT2 and ILT4. Exposure of the PMA/IFN-gamma-treated 
      U937 cells to increasing concentrations of recombinant HLA-G5 or -G6 (rG5 and 
      rG6) stimulated effects common to the two isoforms. High doses of both 
      significantly decreased interleukin (IL)-10 and dramatically increased 
      transforming growth factor-beta1. Differential effectiveness between the isoforms 
      was demonstrated in dose-response studies, as was differential binding to ILT2 
      and ILT4 in receptor-blocking studies. No effects on production of IL-4, IL-1 
      receptor antagonist, IL-15, tumor necrosis factor alpha, IL-1beta, or IL-6 were 
      observed. Collectively, the results are consistent with the postulate that 
      environmental programming of decidual macrophages may be dictated in part by 
      their proximity to soluble HLA-G-producing fetal cytotrophoblast cells.
FAU - McIntire, Ramsey H
AU  - McIntire RH
AD  - Departments of Anatomy and Cell Biology, University of Kansas Medical Center, 
      Kansas City, Kansas 66160, USA.
FAU - Morales, Pedro J
AU  - Morales PJ
FAU - Petroff, Margaret G
AU  - Petroff MG
FAU - Colonna, Marco
AU  - Colonna M
FAU - Hunt, Joan S
AU  - Hunt JS
LA  - eng
GR  - HD26429/HD/NICHD NIH HHS/United States
GR  - HD33994/HD/NICHD NIH HHS/United States
GR  - HD35859/HD/NICHD NIH HHS/United States
GR  - HD39878/HD/NICHD NIH HHS/United States
GR  - P20 RR16475/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20040930
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Antigens, CD)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (LILRB1 protein, human)
RN  - 0 (LILRB2 protein, human)
RN  - 0 (Leukocyte Immunoglobulin-like Receptor B1)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (TGFB1 protein, human)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 130068-27-8 (Interleukin-10)
RN  - 82115-62-6 (Interferon-gamma)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Antigens, CD/immunology/metabolism
MH  - Cell Differentiation/drug effects/immunology
MH  - Dose-Response Relationship, Drug
MH  - Down-Regulation/drug effects/immunology
MH  - Female
MH  - HLA Antigens/genetics/*immunology/pharmacology
MH  - HLA-G Antigens
MH  - Histocompatibility Antigens Class I/genetics/*immunology/pharmacology
MH  - Humans
MH  - Immune Tolerance/immunology
MH  - Interferon-gamma/pharmacology
MH  - Interleukin-10/biosynthesis
MH  - Leukocyte Immunoglobulin-like Receptor B1
MH  - Macrophages/drug effects/*immunology/metabolism
MH  - Membrane Glycoproteins
MH  - Monocytes/drug effects/*immunology/metabolism
MH  - Pregnancy
MH  - Protein Binding/drug effects/immunology
MH  - Protein Isoforms/genetics/metabolism/pharmacology
MH  - Receptors, Immunologic/immunology/metabolism
MH  - Recombinant Fusion Proteins/genetics/immunology/pharmacology
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Transforming Growth Factor beta/*biosynthesis
MH  - Transforming Growth Factor beta1
MH  - U937 Cells
EDAT- 2004/10/02 05:00
MHDA- 2005/01/26 09:00
CRDT- 2004/10/02 05:00
PHST- 2004/10/02 05:00 [pubmed]
PHST- 2005/01/26 09:00 [medline]
PHST- 2004/10/02 05:00 [entrez]
AID - jlb.0604337 [pii]
AID - 10.1189/jlb.0604337 [doi]
PST - ppublish
SO  - J Leukoc Biol. 2004 Dec;76(6):1220-8. doi: 10.1189/jlb.0604337. Epub 2004 Sep 30.