PMID- 15461929
OWN - NLM
STAT- MEDLINE
DCOM- 20041129
LR  - 20191109
IS  - 0898-2104 (Print)
IS  - 0898-2104 (Linking)
VI  - 14
IP  - 1-2
DP  - 2004
TI  - Transendothelial movement of liposomes in vitro mediated by cancer cells, 
      neutrophils or histamine.
PG  - 1-25
AB  - A two-chamber culture system has been used to examine the ability of small 
      liposomes to cross an endothelial cell barrier in response to various stimuli. 
      Transendothelial transit of liposomes was almost negligible in the presence of 
      intact, healthy endothelial cells (EC). Addition of histamine induced a 
      concentration-dependent increase in the movement of liposomes across the EC 
      monolayer. In the presence of polymorphonuclear neutrophils (PMNs), migrating in 
      response to a chemotactic gradient of N-Formil-Met-Leu-Phe (fMLP), both liposomes 
      and IgG crossed EC monolayer by a paracellular pathway, largely independent of an 
      association with the PMNs. The presence of cancer cell, growing in the lower 
      chamber or the presence of cancer cell-conditioned media, also resulted in the 
      passage of liposome across the EC. We conclude that EC monolayers are 
      sufficiently disrupted by several physiologically relevant stimuli to allow for 
      the transendothelial passage of liposomes. These results have important 
      implications for the therapeutic use of liposome in the treatment of cancer or 
      other inflammatory processes.
FAU - Antohe, Felicia
AU  - Antohe F
AD  - Institute of Cellular Biology and Pathology N. Simionescu, Bucharest, Romania.
FAU - Lin, Lei
AU  - Lin L
FAU - Kao, Grace Y
AU  - Kao GY
FAU - Poznansky, Mark J
AU  - Poznansky MJ
FAU - Allen, Theresa M
AU  - Allen TM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Liposome Res
JT  - Journal of liposome research
JID - 9001952
RN  - 0 (Collagen Type I)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Liposomes)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 820484N8I3 (Histamine)
SB  - IM
MH  - Animals
MH  - Brain Neoplasms/*pathology
MH  - Cattle
MH  - Cell Culture Techniques/methods
MH  - Cell Line, Tumor
MH  - Cell Membrane Permeability/drug effects
MH  - Chemotaxis, Leukocyte/drug effects
MH  - Collagen Type I/metabolism
MH  - Colonic Neoplasms/*pathology
MH  - Culture Media, Conditioned/pharmacology
MH  - Endothelium, Vascular/*metabolism/physiology/ultrastructure
MH  - Histamine/*pharmacology
MH  - Humans
MH  - Immunoglobulin G/pharmacology
MH  - *Liposomes
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/drug effects/*physiology/ultrastructure
EDAT- 2004/10/06 09:00
MHDA- 2004/12/16 09:00
CRDT- 2004/10/06 09:00
PHST- 2004/10/06 09:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/10/06 09:00 [entrez]
AID - 120039660 [pii]
AID - 10.1081/lpr-120039660 [doi]
PST - ppublish
SO  - J Liposome Res. 2004;14(1-2):1-25. doi: 10.1081/lpr-120039660.