PMID- 1546368
OWN - NLM
STAT- MEDLINE
DCOM- 19920416
LR  - 20191021
IS  - 0740-7750 (Print)
IS  - 0740-7750 (Linking)
VI  - 18
IP  - 1
DP  - 1992 Jan
TI  - Radiation fusion hybrids for human chromosomes 3 and X generated at various 
      irradiation doses.
PG  - 33-44
AB  - We have used a gamma-irradiation (2.5-25 krads) cell fusion procedure to generate 
      human-hamster somatic cell hybrids (IHB, irradiated human fragments in B14-150 
      cells), retaining small fragments derived from human chromosomes 3 and X. By 
      using Alu-element mediated PCR amplification and dot-blot hybridization with 
      human alphoid or total human DNA as probes, 86 positive hybrids were identified 
      and selected for further analysis. Nonisotopic fluorescence in situ hybridization 
      (FISH) with human DNA in a set of eight hybrids demonstrated the presence of from 
      one to eight human fragments per cell independent of irradiation dose. In 
      contrast, a significant dose-dependent variation of fragment sizes was shown in 
      the analysis of the 86 hybrids with markers previously mapped to 3p (seven 
      markers) and to Xq (21 markers). Using the Xq27-28 region as a model, 40% of the 
      hybrids generated at 5 krads or less were found to have retained fragments in the 
      range of 3-30 Mb, 10% retained the whole chromosome arm, and the remaining 50% 
      retained fragments of less than 2-3 Mb. The proportion of fragments of 3 Mb or 
      larger decreased rapidly at higher irradiation doses and was very low (less than 
      6%) in hybrids generated at 25 krads. Upon further characterization, the 86 
      hybrids analyzed here will provide a mapping panel for the entire chromosomes 3 
      and X with an estimated resolution in the range of 1-2 Mb on average, a size 
      range amenable to PFGE and YAC contig mapping.
FAU - Siden, T S
AU  - Siden TS
AD  - Department of Molecular Genetics, Wallenberg Laboratory, University of Lund, 
      Sweden.
FAU - Kumlien, J
AU  - Kumlien J
FAU - Schwartz, C E
AU  - Schwartz CE
FAU - Rohme, D
AU  - Rohme D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Somat Cell Mol Genet
JT  - Somatic cell and molecular genetics
JID - 8403568
RN  - 0 (DNA Probes)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - CHO Cells/radiation effects
MH  - Cell Fusion/radiation effects
MH  - Chromosomes, Human, Pair 3/*radiation effects
MH  - Cloning, Molecular/radiation effects
MH  - Cricetinae
MH  - DNA Probes
MH  - Dose-Response Relationship, Radiation
MH  - Genetic Markers/radiation effects
MH  - Humans
MH  - Hybrid Cells/*radiation effects
MH  - Molecular Sequence Data
MH  - Nucleic Acid Hybridization
MH  - Polymerase Chain Reaction
MH  - X Chromosome/*radiation effects
EDAT- 1992/01/01 00:00
MHDA- 1992/01/01 00:01
CRDT- 1992/01/01 00:00
PHST- 1992/01/01 00:00 [pubmed]
PHST- 1992/01/01 00:01 [medline]
PHST- 1992/01/01 00:00 [entrez]
AID - 10.1007/BF01233447 [doi]
PST - ppublish
SO  - Somat Cell Mol Genet. 1992 Jan;18(1):33-44. doi: 10.1007/BF01233447.