PMID- 15465655
OWN - NLM
STAT- MEDLINE
DCOM- 20050225
LR  - 20131121
IS  - 0887-2333 (Print)
IS  - 0887-2333 (Linking)
VI  - 18
IP  - 6
DP  - 2004 Dec
TI  - The use of precision-cut liver slices from male Wistar rats as a tool to study 
      age related changes in CYP3A induction and in formation of paracetamol 
      conjugates.
PG  - 879-85
AB  - Precision-cut liver slices (PCLS) offer a lot of advantages because all 
      heterogeneity and cell-cell interactions within the original tissue matrix are 
      maintained. This in vitro model was used to study the effect of ageing on certain 
      aspects of drug metabolism and liver function in young (3 months), adult (9 
      months) and old (24 months) Wistar male rats. Protein synthesis, an important 
      liver function, was not modified in young, adult and old rats, suggesting that 
      ageing does not impair liver functionality but it affects some specific targets. 
      Among them, a decrease in total P450 in liver microsomes and the loss of CYP3A23 
      inducibility in PCLS were clearly observed in old rats as compared to adult rats. 
      Finally, the amount of total paracetamol conjugates was not modified between 9 
      and 24 months but in old rats, sulfoconjugation of paracetamol, its major route 
      of elimination, was decreased.
FAU - Wauthier, Valerie
AU  - Wauthier V
AD  - Unite de Pharmacocinetique, Metabolisme, Nutrition, et Toxicologie (PMNT), 
      Departement des Sciences Pharmaceutiques, Universite Catholique de Louvain, 73 
      Avenue Mounier, 1200 Bruxelles, Belgium.
FAU - Verbeeck, Roger K
AU  - Verbeeck RK
FAU - Buc Calderon, Pedro
AU  - Buc Calderon P
LA  - eng
PT  - Journal Article
PL  - England
TA  - Toxicol In Vitro
JT  - Toxicology in vitro : an international journal published in association with 
      BIBRA
JID - 8712158
RN  - 0 (Analgesics, Non-Narcotic)
RN  - 362O9ITL9D (Acetaminophen)
RN  - EC 1.14.14.1 (Aryl Hydrocarbon Hydroxylases)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP3A)
RN  - EC 1.5.- (Oxidoreductases, N-Demethylating)
SB  - IM
MH  - Acetaminophen/metabolism/*toxicity
MH  - Aging/*physiology
MH  - Analgesics, Non-Narcotic/metabolism/*toxicity
MH  - Animals
MH  - Aryl Hydrocarbon Hydroxylases/*biosynthesis
MH  - Cytochrome P-450 CYP3A
MH  - Enzyme Induction
MH  - Liver/drug effects/*enzymology/growth & development
MH  - Microtomy
MH  - Oxidoreductases, N-Demethylating/*biosynthesis
MH  - Rats
MH  - Rats, Wistar
EDAT- 2004/10/07 09:00
MHDA- 2005/02/26 09:00
CRDT- 2004/10/07 09:00
PHST- 2003/12/22 00:00 [received]
PHST- 2004/04/28 00:00 [accepted]
PHST- 2004/10/07 09:00 [pubmed]
PHST- 2005/02/26 09:00 [medline]
PHST- 2004/10/07 09:00 [entrez]
AID - S0887233304000918 [pii]
AID - 10.1016/j.tiv.2004.04.013 [doi]
PST - ppublish
SO  - Toxicol In Vitro. 2004 Dec;18(6):879-85. doi: 10.1016/j.tiv.2004.04.013.