PMID- 15468156
OWN - NLM
STAT- MEDLINE
DCOM- 20050308
LR  - 20061115
IS  - 8755-1039 (Print)
IS  - 1097-0339 (Linking)
VI  - 31
IP  - 5
DP  - 2004 Nov
TI  - The study of cytopathological aspects induced by human cytomegalovirus infection.
PG  - 289-93
AB  - In cytological examination, human cytomegalovirus (HCMV) infection can not be 
      implied unless typical HCMV-infected cells like owl's-eye cells are present. 
      However, such cells are not always observed in HCMV-infection cases. The aim of 
      our study is to establish the cytopathological features induced by HCMV. In vitro 
      transfection and fluorescence in situ hybridization (FISH) were performed on 
      human embryo lung (HEL) cells. Marked cellular aggregation was observed at 6-hr 
      postinfection (hpi). Multinucleated cells, giant cells, and, particularly, small 
      vacuoles were present in the nuclei or cytoplasm before the appearance of 
      inclusion bodies. However, molding and ground glass in nuclei were absent. Cell 
      clusters displayed round cytoplasm, dispersed later, and showed anisocytosis. All 
      features occurred before 48 hpi when the owl's-eye cell appeared. In FISH, the 
      positive signal highlighted viral particles that became predominant and localized 
      in nuclei. These cytological aspects are dependent on viral replication and 
      contribute to the cytological detection of HCMV infection.
FAU - Takeuchi, Takako
AU  - Takeuchi T
AD  - Department of Pathology, Shimonosekishi-ishikai Hospital, Shimonoseki, Japan.
FAU - Fujii, Akiko
AU  - Fujii A
FAU - Okumiya, Toshika
AU  - Okumiya T
FAU - Watabe, Shoji
AU  - Watabe S
FAU - Ishikawa, Toshizo
AU  - Ishikawa T
FAU - Umeda, Akiko
AU  - Umeda A
FAU - Masuda, Michiyoshi
AU  - Masuda M
FAU - Takeuchi, Hiroaki
AU  - Takeuchi H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Diagn Cytopathol
JT  - Diagnostic cytopathology
JID - 8506895
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Cell Aggregation
MH  - Cells, Cultured
MH  - Cytodiagnosis/*methods
MH  - Cytomegalovirus/genetics/*physiology
MH  - Cytomegalovirus Infections/*pathology/virology
MH  - DNA, Viral/analysis
MH  - Giant Cells/pathology/virology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Inclusion Bodies/pathology/virology
MH  - Lung/embryology/*pathology/virology
MH  - Microscopy, Fluorescence
MH  - Time Factors
MH  - Transfection
EDAT- 2004/10/07 09:00
MHDA- 2005/03/09 09:00
CRDT- 2004/10/07 09:00
PHST- 2004/10/07 09:00 [pubmed]
PHST- 2005/03/09 09:00 [medline]
PHST- 2004/10/07 09:00 [entrez]
AID - 10.1002/dc.20126 [doi]
PST - ppublish
SO  - Diagn Cytopathol. 2004 Nov;31(5):289-93. doi: 10.1002/dc.20126.