PMID- 15468172
OWN - NLM
STAT- MEDLINE
DCOM- 20050412
LR  - 20061115
IS  - 0270-4137 (Print)
IS  - 0270-4137 (Linking)
VI  - 63
IP  - 1
DP  - 2005 Apr 1
TI  - Establishment and characterization of a human primary prostate carcinoma cell 
      line, HH870.
PG  - 91-103
AB  - BACKGROUND: Development of new therapeutic modalities for human prostate 
      carcinoma has been impeded by a lack of adequate in vitro and in vivo models. 
      Most in vitro studies have been carried out using a limited number of human 
      prostate cancer cell lines that are mostly derived from metastatic tumors sites 
      or are immortalized. METHODS: Characterization of the prostate cancer cell line, 
      HH870, included description of morphology, determination of doubling time, 
      response to androgens, immunocytochemistry, and immunoblotting of proteins known 
      to be associated with prostate carcinoma, karyotyping, fluorescence in situ 
      hybridization (FISH), DNA profiling, and growth as xenograft in athymic rodents. 
      RESULTS: HH870 expresses various epithelial marker antigens that correlate with 
      known basic immunostaining profiles of prostate adenocarcinoma, although the cell 
      line does not express PSA, PSMA, or PAP. HH870 exhibits complex chromosomal 
      abnormalities and harbors no immortalizing HPV, BKV, JCV, and SV40 DNA. 
      CONCLUSIONS: We report the successful establishment and characterization of a new 
      long-term primary human prostate tumor cell line HH870.
CI  - Copyright (c) 2004 Wiley-Liss, Inc.
FAU - Selvan, Senthamil R
AU  - Selvan SR
AD  - Patty and George Hoag Cancer Center, Newport Beach, California 92663, USA. 
      sselvan@hoaghospital.org
FAU - Cornforth, Andrew N
AU  - Cornforth AN
FAU - Rao, Nagesh P
AU  - Rao NP
FAU - Reid, Yvonne A
AU  - Reid YA
FAU - Schiltz, Patric M
AU  - Schiltz PM
FAU - Liao, Ray P
AU  - Liao RP
FAU - Price, David T
AU  - Price DT
FAU - Heinemann, F Scott
AU  - Heinemann FS
FAU - Dillman, Robert O
AU  - Dillman RO
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Prostate
JT  - The Prostate
JID - 8101368
RN  - 0 (Androgens)
SB  - IM
MH  - Androgens/metabolism
MH  - Animals
MH  - Cell Culture Techniques/*methods
MH  - Cell Division
MH  - Cell Line, Tumor/*cytology/transplantation
MH  - Chromosome Aberrations
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mice
MH  - Mice, Nude
MH  - Neoplasm Transplantation
MH  - Prostatic Neoplasms/*pathology
EDAT- 2004/10/07 09:00
MHDA- 2005/04/13 09:00
CRDT- 2004/10/07 09:00
PHST- 2004/10/07 09:00 [pubmed]
PHST- 2005/04/13 09:00 [medline]
PHST- 2004/10/07 09:00 [entrez]
AID - 10.1002/pros.20162 [doi]
PST - ppublish
SO  - Prostate. 2005 Apr 1;63(1):91-103. doi: 10.1002/pros.20162.