PMID- 15469870
OWN - NLM
STAT- MEDLINE
DCOM- 20050301
LR  - 20131121
IS  - 0009-8981 (Print)
IS  - 0009-8981 (Linking)
VI  - 349
IP  - 1-2
DP  - 2004 Nov
TI  - Gene analysis of the glucocorticoid receptor alpha in Alzheimer's disease.
PG  - 167-72
AB  - BACKGROUND: In Alzheimer's disease (AD), the hypothalamic-pituitary-adrenal (HPA) 
      axis is hyperactive and the sensitivity to dexamethasone is decreased, suggesting 
      a possible involvement of glucocorticoid receptor alpha (GRalpha) defects in the 
      aetiopathology of the disease. METHODS: We, therefore, searched for the presence 
      of mutations in the human GRalpha (hGRalpha) gene, focusing on the 
      hormone-binding domain due to its importance in mediating glucocorticoids' 
      effects. RNA isolated from peripheral blood mononuclear cells (PBMCs) of 15 
      patients with Alzheimer's disease and 20 healthy individuals was subjected to 
      reverse transcription-polymerase chain reaction amplification (RT-PCR) analysis 
      followed by denaturing gradient gel electrophoresis (DGGE). RESULTS: No mutations 
      could be detected in the region of the hGRalpha gene examined. CONCLUSIONS: We 
      conclude that the hormone-binding domain of GRalpha is not altered in Alzheimer's 
      disease and molecular defects in other gene regions of the GRalpha or in its 
      isoform GRbeta warrant further investigation in Alzheimer's disease.
FAU - Tsolakidou, Amalia F
AU  - Tsolakidou AF
AD  - Department of Biological Chemistry, Medical School, University of Athens, 75 
      Mikras Asias Street, GR-115 27 Goudi, Athens, Greece.
FAU - Coulocheri, Stavroula A
AU  - Coulocheri SA
FAU - Trikkas, George
AU  - Trikkas G
FAU - Moutsatsou, Paraskevi
AU  - Moutsatsou P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Clin Chim Acta
JT  - Clinica chimica acta; international journal of clinical chemistry
JID - 1302422
RN  - 0 (Hormones)
RN  - 0 (Ligands)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 0 (glucocorticoid receptor alpha)
RN  - WI4X0X7BPJ (Hydrocortisone)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Alzheimer Disease/*genetics
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Female
MH  - Hormones/metabolism
MH  - Humans
MH  - Hydrocortisone/metabolism
MH  - Ligands
MH  - Male
MH  - Middle Aged
MH  - Protein Denaturation
MH  - Receptors, Glucocorticoid/*genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2004/10/08 09:00
MHDA- 2005/03/02 09:00
CRDT- 2004/10/08 09:00
PHST- 2004/03/15 00:00 [received]
PHST- 2004/06/14 00:00 [revised]
PHST- 2004/06/17 00:00 [accepted]
PHST- 2004/10/08 09:00 [pubmed]
PHST- 2005/03/02 09:00 [medline]
PHST- 2004/10/08 09:00 [entrez]
AID - S0009-8981(04)00323-7 [pii]
AID - 10.1016/j.cccn.2004.06.014 [doi]
PST - ppublish
SO  - Clin Chim Acta. 2004 Nov;349(1-2):167-72. doi: 10.1016/j.cccn.2004.06.014.