PMID- 15469885 OWN - NLM STAT- MEDLINE DCOM- 20050419 LR - 20060421 IS - 0169-328X (Print) IS - 0169-328X (Linking) VI - 129 IP - 1-2 DP - 2004 Oct 22 TI - Effects of adenoviral vector-mediated BDNF expression on the bulbectomy-induced apoptosis of olfactory receptor neurons. PG - 88-95 AB - The expression of adenoviral vector (Ad)-mediated lacZ and brain-derived neurotrophic factor (BDNF) in mouse olfactory epithelium (OE) was examined, and the effect of BDNF on the survival of the bulbectomized OE was evaluated. A recombinant adenovirus, Ax1CAlacZ, was administrated into the mouse OE after bulbectomy, and the expression of a transferred E. coli beta-galactosidase (beta-gal) gene was confirmed by X-gal staining. The expression and effects of exogenous BDNF in the OE after bulbectomy were examined using immunohistochemistry and the TUNEL method. The adenoviral vector-mediated expression of beta-gal in the mouse OE was detectable for up to 14 days after bulbectomy in vivo. The Ad-mediated expression of BDNF was also observed in the OE after bulbectomy. Exogenously induced BDNF suppressed the degenerative changes of bulbectomized OE. TUNEL staining indicated that the exogenous BDNF enhanced the survival of the bulbectomized OE by inhibiting apoptosis. Ad-mediated expression of BDNF in the mouse nasal mucosa alleviated degenerative changes in bulbectomized OE. Ad-mediated transfer of neurotrophic factors might be applicable in the treatment of olfactory disorders. FAU - Isoyama, Kyoko AU - Isoyama K AD - Department of Otorhinolaryngology (J2), Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chiba 260-8670, Japan. FAU - Nagata, Hiroshi AU - Nagata H FAU - Shino, Yuji AU - Shino Y FAU - Isegawa, Naohisa AU - Isegawa N FAU - Arimoto, Yukiko AU - Arimoto Y FAU - Koda, Masao AU - Koda M FAU - Kumahara, Keiichiro AU - Kumahara K FAU - Okamoto, Yoshitaka AU - Okamoto Y FAU - Shirasawa, Hiroshi AU - Shirasawa H LA - eng PT - Journal Article PL - Netherlands TA - Brain Res Mol Brain Res JT - Brain research. Molecular brain research JID - 8908640 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Recombinant Fusion Proteins) SB - IM MH - Adenoviridae/*genetics/metabolism MH - Animals MH - Apoptosis/*physiology MH - Brain-Derived Neurotrophic Factor/genetics/*metabolism MH - Cell Survival MH - Female MH - Genes, Reporter MH - *Genetic Vectors MH - HeLa Cells MH - Humans MH - In Situ Nick-End Labeling MH - Mice MH - Mice, Inbred BALB C MH - Olfactory Bulb/anatomy & histology/*surgery MH - Olfactory Receptor Neurons/cytology/*physiology MH - Recombinant Fusion Proteins/genetics/metabolism EDAT- 2004/10/08 09:00 MHDA- 2005/04/20 09:00 CRDT- 2004/10/08 09:00 PHST- 2004/06/16 00:00 [accepted] PHST- 2004/10/08 09:00 [pubmed] PHST- 2005/04/20 09:00 [medline] PHST- 2004/10/08 09:00 [entrez] AID - S0169-328X(04)00305-5 [pii] AID - 10.1016/j.molbrainres.2004.06.031 [doi] PST - ppublish SO - Brain Res Mol Brain Res. 2004 Oct 22;129(1-2):88-95. doi: 10.1016/j.molbrainres.2004.06.031.