PMID- 15471568
OWN - NLM
STAT- MEDLINE
DCOM- 20041129
LR  - 20161126
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1665
IP  - 1-2
DP  - 2004 Oct 11
TI  - Influence of lipid chain unsaturation on membrane-bound melittin: a fluorescence 
      approach.
PG  - 29-39
AB  - Melittin, a cationic hemolytic peptide, is intrinsically fluorescent due to the 
      presence of a single functionally important tryptophan residue. The organization 
      of membrane-bound melittin is dependent on the physical state and composition of 
      membranes. In particular, polyunsaturated lipids have been shown to modulate the 
      membrane-disruptive action of melittin. Phospholipids with polyunsaturated acyl 
      chains are known to modulate a number of physical properties of membranes and 
      play an important role in regulating structure and function of membrane proteins. 
      In this study, we have used melittin to address the influence of unsaturated 
      lipids in modulating lipid-protein interactions. Our results show that 
      fluorescence parameters such as intensity, emission maximum, polarization, 
      lifetime and acrylamide quenching of melittin incorporated in membranes are 
      dependent on the degree of unsaturation of lipids in membranes. Importantly, 
      melittin in membranes composed of various unsaturated lipids shows red edge 
      excitation shift (REES) implying that melittin is localized in a motionally 
      restricted region in membranes. The extent of REES was found to increase 
      drastically in membranes with increasing unsaturation, especially when the lipids 
      contained more than two double bonds. In addition, increasing unsaturation in 
      membranes causes a considerable change in the secondary structure of 
      membrane-bound melittin. Taken together, our results assume significance in the 
      overall context of the role of unsaturated lipids in membranes in the 
      organization and function of membrane proteins and membrane-active peptides.
FAU - Raghuraman, H
AU  - Raghuraman H
AD  - Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
FAU - Chattopadhyay, Amitabha
AU  - Chattopadhyay A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Fatty Acids, Unsaturated)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Liposomes)
RN  - 0 (Membrane Lipids)
RN  - 0 (Membrane Proteins)
RN  - 0 (Phosphatidylcholines)
RN  - 20449-79-0 (Melitten)
SB  - IM
MH  - Animals
MH  - Fatty Acids, Unsaturated/*chemistry
MH  - Fluorescent Dyes
MH  - Liposomes
MH  - Melitten/*chemistry
MH  - Membrane Lipids/*chemistry
MH  - Membrane Proteins/*chemistry
MH  - Phosphatidylcholines/chemistry
MH  - Spectrometry, Fluorescence
EDAT- 2004/10/09 09:00
MHDA- 2004/12/16 09:00
CRDT- 2004/10/09 09:00
PHST- 2004/02/25 00:00 [received]
PHST- 2004/06/08 00:00 [revised]
PHST- 2004/06/22 00:00 [accepted]
PHST- 2004/10/09 09:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/10/09 09:00 [entrez]
AID - S0005-2736(04)00154-3 [pii]
AID - 10.1016/j.bbamem.2004.06.008 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2004 Oct 11;1665(1-2):29-39. doi: 
      10.1016/j.bbamem.2004.06.008.