PMID- 15472297
OWN - NLM
STAT- MEDLINE
DCOM- 20041223
LR  - 20191210
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 42
IP  - 10
DP  - 2004 Oct
TI  - Detection of Paracoccidioides brasiliensis gp70 circulating antigen and follow-up 
      of patients undergoing antimycotic therapy.
PG  - 4480-6
AB  - Paracoccidioidomycosis (PCM), one of the most important systemic mycoses in 
      Central and South America, is caused by the dimorphic fungus Paracoccidioides 
      brasiliensis and has a high prevalence in Brazil. Glycoproteins of 43 and 70 kDa 
      are the main antigenic compounds of P. brasiliensis and are recognized by Western 
      blotting by 100 and 96% of PCM patient sera, respectively. In the present study, 
      an inhibition enzyme-linked immunosorbent assay (ELISA) was used to detect gp70 
      in different biological samples from patients with PCM. gp70 was detected in 
      98.76% of 81 serum samples, with an average concentration of 8.19 microg/ml. The 
      test was positive for 100% of the patients with the acute and chronic unifocal 
      forms of PCM and 98.43% of the patients with the multifocal chronic form, with 
      average concentrations of 11.86, 4.83, and 7.87 microg/ml, respectively. 
      Bronchoalveolar lavage fluid from 23 patients with pulmonary unifocal PCM and 14 
      samples of cerebrospinal fluid from patients with neurological PCM were also 
      tested for gp70 detection, with the test showing 100% sensitivity and 100% 
      specificity, with mean gp70 concentrations of 7.5 and 6.78 microg/ml, 
      respectively. To investigate the potential of gp70 detection by inhibition ELISA 
      for the follow-up of PCM patients during antimycotic therapy with itraconazole 
      (ITZ), the sera of 23 patients presenting with the chronic multifocal form of PCM 
      were monitored at regular intervals of 1 month for 12 months. The results showed 
      a decrease in circulating gp70 levels during treatment which paralleled the 
      reduction in anti-P. brasiliensis antibody levels. The detection of P. 
      brasiliensis gp70 from the biological fluids of patients suspected of having PCM 
      proved to be a promising method for diagnosing infection and evaluating the 
      efficacy of ITZ treatment.
FAU - da Silva, Silvia Helena Marques
AU  - da Silva SH
AD  - Department of Microbiology, Immunology and Parasitology, Federal University of 
      Sao Paulo, Sao Paulo, SP, Brazil.
FAU - Grosso, Daniela de Mattos
AU  - Grosso Dde M
FAU - Lopes, Jose Daniel
AU  - Lopes JD
FAU - Colombo, Arnaldo Lopes
AU  - Colombo AL
FAU - Blotta, Maria Heloisa Souza Lima
AU  - Blotta MH
FAU - Queiroz-Telles, Flavio
AU  - Queiroz-Telles F
FAU - de Camargo, Zoilo Pires
AU  - de Camargo ZP
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Antifungal Agents)
RN  - 0 (Antigens, Fungal)
RN  - 304NUG5GF4 (Itraconazole)
SB  - IM
MH  - Adolescent
MH  - Antifungal Agents/*therapeutic use
MH  - Antigens, Fungal/*blood
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Female
MH  - Humans
MH  - Itraconazole/*therapeutic use
MH  - Male
MH  - Middle Aged
MH  - Paracoccidioides/immunology/*isolation & purification
MH  - Paracoccidioidomycosis/*diagnosis/*drug therapy/microbiology
PMC - PMC522319
EDAT- 2004/10/09 09:00
MHDA- 2004/12/24 09:00
PMCR- 2004/10/01
CRDT- 2004/10/09 09:00
PHST- 2004/10/09 09:00 [pubmed]
PHST- 2004/12/24 09:00 [medline]
PHST- 2004/10/09 09:00 [entrez]
PHST- 2004/10/01 00:00 [pmc-release]
AID - 42/10/4480 [pii]
AID - 0504-04 [pii]
AID - 10.1128/JCM.42.10.4480-4486.2004 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2004 Oct;42(10):4480-6. doi: 10.1128/JCM.42.10.4480-4486.2004.