PMID- 15472509
OWN - NLM
STAT- MEDLINE
DCOM- 20041116
LR  - 20231213
IS  - 1078-0998 (Print)
IS  - 1078-0998 (Linking)
VI  - 10
IP  - 5
DP  - 2004 Sep
TI  - Characterization and distribution of colonic dendritic cells in Crohn's disease.
PG  - 504-12
AB  - Dendritic cells (DCs) are thought to play an important role in the pathogenesis 
      of autoimmune inflammation, including Crohn's disease (CD). We investigated the 
      distribution and state of maturation of DCs in the colon in relation to the 
      severity of inflammation and therapy. Using archival specimens from colonic 
      resections in 19 pediatric patients with CD and 14 controls, we identified and 
      characterized the DCs within the lamina propria, submucosa, and muscularis 
      compartments using morphologic and quantitative immunohistochemical methods. The 
      distribution of CD11c+CD83+CD68+DC-SIGN+ and immature CD11c+CD83-CD68-DC-SIGN+ 
      DCs within the different compartments varied according to the presence or absence 
      of CD as well as to the severity of inflammation and systemic corticoid 
      treatment. Immature DCs were only found in non-inflamed control colonic tissue. 
      Marked reductions (60% and 30%) in total CD11c and CD83 DC numbers were observed 
      in CD tissue samples compared with controls (P < 0.05). CD samples from patients 
      on corticosteroid therapy were significantly more depleted than in tissue from 
      untreated patients or those on other drugs. Colonic tissue with severe 
      inflammation had reduced numbers of CD11c+ and CD83+ DCs in the lamina propria 
      and submucosal compartments (80% and 76% for CD11c; 75% and 76% for CD83, 
      respectively, P < 0.05), with a concomitant increase (525% for CD11c and 700% for 
      CD83 P < 0.05) of DCs in the muscularis compartment, compared to moderately 
      inflamed and non-inflamed CD tissue. Our data suggest that an imbalance in 
      intestinal DC subpopulations may play a role in the initiation and/or the 
      maintenance of chronic inflammation in CD. Corticosteroid therapy is associated 
      with colonic DC depletion.
CI  - Copyright 2004 Lippincott Williams & Wilkins
FAU - Silva, Manuel A
AU  - Silva MA
AD  - Mucosal Immunology Laboratory, Division of Gastroenterology, Hepatology & 
      Nutrition, Sainte-Justine Hospital Research Centre, Department of Paediatrics, 
      University of Montreal, Quebec, Canada H3TICS.
FAU - Lopez, Carolina B
AU  - Lopez CB
FAU - Riverin, Francine
AU  - Riverin F
FAU - Oligny, Luc
AU  - Oligny L
FAU - Menezes, Jose
AU  - Menezes J
FAU - Seidman, Ernest G
AU  - Seidman EG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Inflamm Bowel Dis
JT  - Inflammatory bowel diseases
JID - 9508162
RN  - 0 (Adrenal Cortex Hormones)
RN  - 0 (Antigens, CD)
RN  - 0 (CD11 Antigens)
RN  - 0 (Immunoglobulins)
RN  - 0 (Membrane Glycoproteins)
SB  - IM
MH  - Adolescent
MH  - Adrenal Cortex Hormones/therapeutic use
MH  - Antigens, CD
MH  - CD11 Antigens/analysis
MH  - Case-Control Studies
MH  - Cell Differentiation
MH  - Crohn Disease/drug therapy/*immunology/*pathology
MH  - *Dendritic Cells
MH  - Female
MH  - Humans
MH  - Immunoglobulins/analysis
MH  - Inflammation/pathology
MH  - Male
MH  - Membrane Glycoproteins/analysis
MH  - Severity of Illness Index
MH  - CD83 Antigen
EDAT- 2004/10/09 09:00
MHDA- 2004/11/17 09:00
CRDT- 2004/10/09 09:00
PHST- 2004/10/09 09:00 [pubmed]
PHST- 2004/11/17 09:00 [medline]
PHST- 2004/10/09 09:00 [entrez]
AID - 00054725-200409000-00003 [pii]
AID - 10.1097/00054725-200409000-00003 [doi]
PST - ppublish
SO  - Inflamm Bowel Dis. 2004 Sep;10(5):504-12. doi: 10.1097/00054725-200409000-00003.