PMID- 15474021
OWN - NLM
STAT- MEDLINE
DCOM- 20041119
LR  - 20181130
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 576
IP  - 1-2
DP  - 2004 Oct 8
TI  - Clustering of cellular prion protein induces ERK1/2 and stathmin phosphorylation 
      in GT1-7 neuronal cells.
PG  - 114-8
AB  - The physiological role of the prion protein is largely unknown. Here, clustering 
      of prion at the surface of GT1-7 cells was observed upon anti-prion antibody 
      treatments. This clustering was associated with a rapid and transient 
      phosphorylation of the mitogen activated protein kinases (MAPKs) extracellular 
      receptor kinases 1 and 2 (ERK1/2), and also of the microtubule-destabilizing 
      protein stathmin at serine 16. The specificity of this antibody-mediated 
      activation was ascertained by its inhibition by prion small interfering RNA. The 
      phosphorylation of ERK1/2 but not that of stathmin was abolished by the MAPK/ERK 
      kinase 1 inhibitor U0126, whereas both signaling pathways were blocked by the 
      specific inhibitor of the epidermal growth factor receptor AG1478, suggesting the 
      likely recruitment of this receptor upon prion clustering.
CI  - Copyright 2004 Federation of European Biochemical Societies
FAU - Monnet, Celine
AU  - Monnet C
AD  - Signalisation et Differenciation Cellulaires dans les Systemes Nerveux et 
      Musculaire, U440 INSERM/UPMC, Institut du Fer a Moulin, 17 rue du Fer a Moulin, 
      75005 Paris, France.
FAU - Gavard, Julie
AU  - Gavard J
FAU - Mege, Rene-Marc
AU  - Mege RM
FAU - Sobel, Andre
AU  - Sobel A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Butadienes)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Microtubule Proteins)
RN  - 0 (Nitriles)
RN  - 0 (Phosphoproteins)
RN  - 0 (PrPC Proteins)
RN  - 0 (Quinazolines)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Stathmin)
RN  - 0 (Stmn1 protein, mouse)
RN  - 0 (Tyrphostins)
RN  - 0 (U 0126)
RN  - 170449-18-0 (RTKI cpd)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/metabolism
MH  - Blotting, Western
MH  - Butadienes/pharmacology
MH  - Cells, Cultured
MH  - Enzyme Inhibitors/pharmacology
MH  - Fluorescent Antibody Technique, Indirect
MH  - Mice
MH  - Microscopy, Confocal
MH  - Microtubule Proteins/*metabolism
MH  - Mitogen-Activated Protein Kinase 1/drug effects/*metabolism
MH  - Mitogen-Activated Protein Kinase 3/drug effects/*metabolism
MH  - Neurons/*physiology
MH  - Nitriles/pharmacology
MH  - Phosphoproteins/*metabolism
MH  - Phosphorylation/drug effects
MH  - PrPC Proteins/*physiology
MH  - Quinazolines
MH  - RNA, Small Interfering/antagonists & inhibitors
MH  - Stathmin
MH  - Tyrphostins/pharmacology
EDAT- 2004/10/12 09:00
MHDA- 2004/12/16 09:00
CRDT- 2004/10/12 09:00
PHST- 2004/08/06 00:00 [received]
PHST- 2004/08/16 00:00 [accepted]
PHST- 2004/10/12 09:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/10/12 09:00 [entrez]
AID - S0014579304011044 [pii]
AID - 10.1016/j.febslet.2004.08.076 [doi]
PST - ppublish
SO  - FEBS Lett. 2004 Oct 8;576(1-2):114-8. doi: 10.1016/j.febslet.2004.08.076.