PMID- 15474069
OWN - NLM
STAT- MEDLINE
DCOM- 20041119
LR  - 20220409
IS  - 0015-0282 (Print)
IS  - 0015-0282 (Linking)
VI  - 82 Suppl 3
DP  - 2004 Oct
TI  - Early embryo-endometrial signaling modulates the regulation of matrix 
      metalloproteinase-3.
PG  - 1029-35
AB  - OBJECTIVE: To examine matrix metalloproteinase-3 (MMP-3) expression in human 
      stromal cell culture after P stimulation and the effect of conditioned medium 
      from human embryo-epithelial cells coculture on its expression and activity. 
      DESIGN: Metabolic and endocrine studies on human tissue. SETTING: In vitro 
      fertilization (i.v.f.) unit and endocrine research unit. PATIENT(S): Infertile 
      patients undergoing endometrial tissue sampling for dating at the luteal phase 
      before i.v.f. INTERVENTION(S): Endometrial sampling and collection of human 
      embryos culture media. MAIN OUTCOME MEASURE(S): Expression and activity of 
      secreted MMP-3 by P-induced stromal cells, and in stromal cells exposed to 
      conditioned medium from embryo-epithelial cell coculture. RESULT(S): Expression 
      and activity of MMP-3 in human stromal cells decrease after P induction. 
      Following incubation of these stromal-derived decidual cells with conditioned 
      medium from embryo-epithelial cell coculture, MMP-3 expression and activity 
      increased in a statistically significant manner. CONCLUSION(S): Progesterone 
      inhibition of MMP-3 expression and its support of endometrial integrity were 
      prevented by local expression of MMP-3 in response to embryonic signaling.
FAU - Lahav-Baratz, Shirly
AU  - Lahav-Baratz S
AD  - Department of Obstetrics and Gynecology, IVF Unit, Carmel Medical Center, Haifa, 
      Israel. lahav@adi.org il
FAU - Shiloh, Hanna
AU  - Shiloh H
FAU - Koifman, Mara
AU  - Koifman M
FAU - Kraiem, Zaki
AU  - Kraiem Z
FAU - Wiener-Megnazi, Zofnat
AU  - Wiener-Megnazi Z
FAU - Ishai, David
AU  - Ishai D
FAU - Dirnfeld, Martha
AU  - Dirnfeld M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Matrix Metalloproteinase Inhibitors)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Adult
MH  - Blastocyst/enzymology
MH  - Cell Differentiation
MH  - Coculture Techniques
MH  - Culture Media, Conditioned/pharmacology
MH  - Culture Techniques
MH  - Decidua/cytology/drug effects/enzymology
MH  - Embryo, Mammalian/*physiology
MH  - Embryonic Development
MH  - Endometrium/cytology/*physiology
MH  - Female
MH  - Humans
MH  - Matrix Metalloproteinase 3/*metabolism
MH  - Matrix Metalloproteinase Inhibitors
MH  - Morula/enzymology
MH  - Progesterone/pharmacology
MH  - Signal Transduction/*physiology
MH  - Stromal Cells/cytology
MH  - Time Factors
EDAT- 2004/10/12 09:00
MHDA- 2004/12/16 09:00
CRDT- 2004/10/12 09:00
PHST- 2003/10/15 00:00 [received]
PHST- 2004/06/01 00:00 [revised]
PHST- 2004/06/01 00:00 [accepted]
PHST- 2004/10/12 09:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/10/12 09:00 [entrez]
AID - S0015-0282(04)01265-8 [pii]
AID - 10.1016/j.fertnstert.2004.06.026 [doi]
PST - ppublish
SO  - Fertil Steril. 2004 Oct;82 Suppl 3:1029-35. doi: 
      10.1016/j.fertnstert.2004.06.026.