PMID- 15474092
OWN - NLM
STAT- MEDLINE
DCOM- 20041119
LR  - 20131121
IS  - 0015-0282 (Print)
IS  - 0015-0282 (Linking)
VI  - 82 Suppl 3
DP  - 2004 Oct
TI  - Tumor necrosis factor-alpha-308 promoter and p53 codon 72 gene polymorphisms in 
      women with leiomyomas.
PG  - 1177-81
AB  - OBJECTIVE: Tumor necrosis factor-alpha (TNF-alpha), a proinflammatory cytokine, 
      plays an important role in the process of autoimmune diseases. p53 is related to 
      the regulation of cell growth and prevention of carcinogenesis. We propose to 
      investigate whether gene polymorphisms for TNF-alpha-308 promoter and p53 could 
      be used as markers of susceptibility in leiomyomas. DESIGN: Prospective basic 
      study. SETTING: Departments of gynecology and genetics in a medical center. 
      PATIENT(S): Group 1: leiomyoma (n = 159); group 2: non-leiomyoma (n = 131). 
      INTERVENTION(S): Genomic DNA was obtained from peripheral leukocyte. The 
      TNF-alpha and p53 gene polymorphisms were amplified by polymerase chain reaction 
      (PCR), enzyme restriction, and electrophoresis. MAIN OUTCOME MEASURE(S): Two gene 
      polymorphisms were identified: [1] the A (cuttable)/G (uncuttable) polymorphisms 
      of the TNF-alpha gene on chromosome 6p21.3; [2] A (cuttable)/P (uncuttable) 
      polymorphisms of the p53 gene on chromosome 17p. Genotype and allelic frequencies 
      in both groups were compared. RESULT(S): Genotype distribution and allele 
      frequency of TNF-alpha gene polymorphism in both groups were significantly 
      different. Proportions of A homozygote/heterozygote/G homozygote for TNF-alpha in 
      both groups were: (group 1) 61%/34.6%/4.4% and (group 2) 81.7%/14.5%/3.8%. 
      Proportions of allele A/G for TNF-alpha in both groups were: (group 1) 
      78.3%/21.7% and (group 2) 88.9%/11.1%. Distributions of p53 polymorphisms in both 
      groups were not different. The proportions of A homozygotes/heterozygotes/P 
      homozygotes for p53 were (group 1) 32.7%/42.1%/25.2% and (group 2) 
      28.2%/48.9%/22.9%. CONCLUSION(S): G homozygote and G allele for TNF-alpha 
      promoter are related to a higher risk of leiomyomas. The p53 codon 72 gene 
      polymorphism is not associated with the susceptibility of leiomyomas.
FAU - Hsieh, Yao-Yuan
AU  - Hsieh YY
AD  - Department of Obstetrics and Gynecology, China Medical University Hospital, 
      Taichung, Taiwan.
FAU - Chang, Chi-Chen
AU  - Chang CC
FAU - Tsai, Fuu-Jen
AU  - Tsai FJ
FAU - Lin, Cheng-Chieh
AU  - Lin CC
FAU - Yeh, Lian-Shun
AU  - Yeh LS
FAU - Tsai, Chang-Hai
AU  - Tsai CH
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
RN  - 0 (Codon)
RN  - 0 (Genetic Markers)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 5Z93L87A1R (Guanine)
RN  - JAC85A2161 (Adenine)
SB  - IM
MH  - Adenine
MH  - Alleles
MH  - Case-Control Studies
MH  - Chromosomes, Human, Pair 17
MH  - Chromosomes, Human, Pair 6
MH  - Codon/*genetics
MH  - Female
MH  - Gene Frequency
MH  - *Genes, p53
MH  - Genetic Markers
MH  - Genetic Predisposition to Disease
MH  - Genotype
MH  - Guanine
MH  - Homozygote
MH  - Humans
MH  - Leiomyoma/*genetics
MH  - *Polymorphism, Single Nucleotide
MH  - *Promoter Regions, Genetic
MH  - Prospective Studies
MH  - Tumor Necrosis Factor-alpha/*genetics
MH  - Uterine Neoplasms/*genetics
EDAT- 2004/10/12 09:00
MHDA- 2004/12/16 09:00
CRDT- 2004/10/12 09:00
PHST- 2003/06/16 00:00 [received]
PHST- 2004/03/01 00:00 [revised]
PHST- 2004/03/01 00:00 [accepted]
PHST- 2004/10/12 09:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/10/12 09:00 [entrez]
AID - S0015-0282(04)01109-4 [pii]
AID - 10.1016/j.fertnstert.2004.03.035 [doi]
PST - ppublish
SO  - Fertil Steril. 2004 Oct;82 Suppl 3:1177-81. doi: 
      10.1016/j.fertnstert.2004.03.035.