PMID- 15474149
OWN - NLM
STAT- MEDLINE
DCOM- 20041124
LR  - 20131121
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 154
IP  - 2
DP  - 2004 Oct 15
TI  - Interphase fluorescence in situ hybridization studies for the detection of 9q34 
      deletions in chronic myelogenous leukemia: a practical approach to clinical 
      diagnosis.
PG  - 138-43
AB  - Chronic myelogenous leukemia (CML) is characterized by the Philadelphia 
      chromosome (Ph) in more than 90% of cases. Recent studies using fluorescence in 
      situ hybridization (FISH) have shown that in a subset of patients with CML, 
      deletions of 9q34 involving the argininosuccinate synthetase region occur at the 
      time of the Philadelphia translocation and are associated with a poor prognosis. 
      We performed interphase FISH studies in 152 cases of CML using a dual-color, 
      dual-fusion probe system with a third probe directed at 9q34. Cytogenetic studies 
      showed a simple (typical) Ph in 124/152 (82%), a cryptic Ph in 11/152 (7%), and a 
      variant Ph chromosome with a complex translocation in 17/152 (11%) of cases. 
      Interphase FISH studies showed single BCR/ABL fusion patterns in 48/152 (32%) of 
      cases. Deletions of 9q34 were observed in 14% of all the cases and were present 
      in 46% of cases with single BCR/ABL fusion pattern. All the 9q34 deletions 
      occurred in cases with single BCR/ABL fusion signal. However, a single-fusion 
      pattern is not specific for 9q34 deletions, and cases should be routinely 
      screened for the presence of this prognostically significant abnormality by using 
      a third probe directed specifically at 9q34.
FAU - Aoun, Patricia
AU  - Aoun P
AD  - Department of Pathology and Microbiology, University of Nebraska Medical Center, 
      Omaha 68198, USA. paoun@unmc.edu
FAU - Wiggins, Michele
AU  - Wiggins M
FAU - Pickering, Diane
AU  - Pickering D
FAU - Foran, James
AU  - Foran J
FAU - Rasheed, Husain
AU  - Rasheed H
FAU - Pavletic, Steve Z
AU  - Pavletic SZ
FAU - Sanger, Warren
AU  - Sanger W
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA Probes)
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Chromosome Banding
MH  - *Chromosome Deletion
MH  - *Chromosomes, Human, Pair 9
MH  - DNA Probes
MH  - Fusion Proteins, bcr-abl
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase
MH  - Karyotyping
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*diagnosis/*genetics
MH  - Philadelphia Chromosome
EDAT- 2004/10/12 09:00
MHDA- 2004/12/16 09:00
CRDT- 2004/10/12 09:00
PHST- 2004/01/05 00:00 [received]
PHST- 2004/02/06 00:00 [revised]
PHST- 2004/02/12 00:00 [accepted]
PHST- 2004/10/12 09:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/10/12 09:00 [entrez]
AID - S0165-4608(04)00090-1 [pii]
AID - 10.1016/j.cancergencyto.2004.02.012 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2004 Oct 15;154(2):138-43. doi: 
      10.1016/j.cancergencyto.2004.02.012.