PMID- 15474463
OWN - NLM
STAT- MEDLINE
DCOM- 20041220
LR  - 20101118
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 324
IP  - 2
DP  - 2004 Nov 12
TI  - Effect of Add1 gene transfer on blood pressure in reciprocal congenic strains of 
      Milan rats.
PG  - 562-8
AB  - Genetic variants of alpha adducin (ADD1) taken alone or in interaction with those 
      of beta (ADD2) and gamma (ADD3) subunits have been associated with primary 
      hypertension in humans and in Milan hypertensive (MHS) rats. In this study, we 
      report the dissection of the individual contribution of each rat Add gene to 
      blood pressure, by congenic substitution mapping. Congenic strains were developed 
      by introgressing Add1, Add2, and Add3 genes (and chr14, chr4, and chr1 associated 
      segments) of MHS in the Milan normotensive rat (MNS) genetic background 
      (MNS.H-Add1, MNS.H-Add2, and MNS.H-Add3) and vice versa (MHS.N-Add1, MHS.N-Add2, 
      and MHS.N-Add3). Systolic blood pressure (SBP) of MNS.H-Add1 rats was 
      significantly higher (+10 mmHg) than that of MNS, whereas SBP of MHS.N-Add1 was 
      significantly lower (-10 mmHg) than that of MHS. The differences account for 43% 
      of the blood pressure differences between MHS and MNS. In contrast, SBPs of Add2 
      and Add3 congenic strains were not different from those of the correspondent 
      recipient parental strain. The fine mapping of chr14 congenic segment supports 
      the identity of blood pressure QTL with Add1 gene.
FAU - Tripodi, Grazia
AU  - Tripodi G
AD  - Prassis-Sigma Tau Research Institute, Settimo Milanese, Milan, Italy. 
      grazia.tripodi@prassis.it
FAU - Florio, Monica
AU  - Florio M
FAU - Ferrandi, Mara
AU  - Ferrandi M
FAU - Modica, Rossana
AU  - Modica R
FAU - Zimdahl, Heike
AU  - Zimdahl H
FAU - Hubner, Norbert
AU  - Hubner N
FAU - Ferrari, Patrizia
AU  - Ferrari P
FAU - Bianchi, Giuseppe
AU  - Bianchi G
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (ADD3 protein, human)
RN  - 0 (CCAAT-Enhancer-Binding Proteins)
RN  - 0 (Calmodulin-Binding Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (SREBF1 protein, human)
RN  - 0 (Srebf1 protein, rat)
RN  - 0 (Sterol Regulatory Element Binding Protein 1)
RN  - 0 (Transcription Factors)
RN  - 0 (adducin)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Animals, Congenic
MH  - Blood Pressure
MH  - CCAAT-Enhancer-Binding Proteins/*genetics
MH  - Calmodulin-Binding Proteins/*genetics/metabolism
MH  - Chromosome Mapping
MH  - Chromosomes/ultrastructure
MH  - Crosses, Genetic
MH  - DNA/metabolism
MH  - DNA-Binding Proteins/*genetics
MH  - Female
MH  - *Gene Transfer Techniques
MH  - Genetic Linkage
MH  - Humans
MH  - Hypertension/genetics/pathology
MH  - Male
MH  - Models, Genetic
MH  - Rats
MH  - Sterol Regulatory Element Binding Protein 1
MH  - Transcription Factors/*genetics
EDAT- 2004/10/12 09:00
MHDA- 2004/12/21 09:00
CRDT- 2004/10/12 09:00
PHST- 2004/09/09 00:00 [received]
PHST- 2004/10/12 09:00 [pubmed]
PHST- 2004/12/21 09:00 [medline]
PHST- 2004/10/12 09:00 [entrez]
AID - S0006-291X(04)02140-0 [pii]
AID - 10.1016/j.bbrc.2004.09.079 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2004 Nov 12;324(2):562-8. doi: 
      10.1016/j.bbrc.2004.09.079.