PMID- 15476173
OWN - NLM
STAT- MEDLINE
DCOM- 20090303
LR  - 20181130
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 21
IP  - 5
DP  - 2004 Oct
TI  - [Detection of ALK gene translocation by fluorescence in situ hybridization in 
      paraffin-embedded anaplastic large cell lymphoma tissue and its significance].
PG  - 470-3
AB  - OBJECTIVE: To compare the value of fluorescence in situ hybridization(FISH) with 
      that of immunohistochemistry in detecting ALK gene translocations and ALK fusion 
      protein in anaplastic large cell lymphoma (ALCL) and investigate the possibility 
      of FISH working on paraffin-embedded ALCL tissue. METHODS: Dual-color FISH and 
      ALK-1 immunohistochemistry were used to detect ALK gene translocation and ALK 
      fusion protein in 22 paraffin-embedded ALCL cases. RESULTS: The digestion time of 
      tissue section was a key-point in the operating of FISH in paraffin-embedded 
      tissue. ALK fusion protein expression was detected with ALK-1 antibody in 12 of 
      the 20 systemic ALCL; it was not detected in 2 primary cutaneous ALCL. The 
      Dual-color FISH results were 100% consonant with the immunohistochemical results. 
      CONCLUSION: (1) Generally, immunohistochemical method is the first choice in 
      detecting ALK gene translocation, but when conditions permit, FISH can be the 
      first choice. (2) By the optimization of experimental conditions, FISH can be 
      successfully performed on paraffin-embedded tissue.
FAU - Li, Hai-yan
AU  - Li HY
AD  - Department of Pathology,West China Hospital,Sichuan University,Chengdu, Sichuan, 
      610041 P.R. China.
FAU - Li, Gan-di
AU  - Li GD
FAU - He, Xiao-jin
AU  - He XJ
FAU - Liu, Wei-ping
AU  - Liu WP
FAU - Zhang, Jie
AU  - Zhang J
FAU - Yang, Wen-xiu
AU  - Yang WX
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - EC 2.7.10.1 (ALK protein, human)
RN  - EC 2.7.10.1 (Anaplastic Lymphoma Kinase)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
SB  - IM
MH  - Anaplastic Lymphoma Kinase
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lymphoma, Large-Cell, Anaplastic/*genetics/pathology
MH  - Paraffin Embedding/methods
MH  - Protein-Tyrosine Kinases/*genetics
MH  - Receptor Protein-Tyrosine Kinases
MH  - Reproducibility of Results
MH  - Translocation, Genetic
EDAT- 2004/10/12 09:00
MHDA- 2009/03/04 09:00
CRDT- 2004/10/12 09:00
PHST- 2004/10/12 09:00 [pubmed]
PHST- 2009/03/04 09:00 [medline]
PHST- 2004/10/12 09:00 [entrez]
AID - 940621119 [pii]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2004 Oct;21(5):470-3.