PMID- 1547653
OWN - NLM
STAT- MEDLINE
DCOM- 19920423
LR  - 20131121
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 13
IP  - 1
DP  - 1992
TI  - Increase in acridine orange (AO) fluorescence intensity of monocytes cultured in 
      plastic tissue culture plates as measured by flow cytometry.
PG  - 103-7
AB  - Although the green-red fluorescence of AO is an accepted measure of DNA-RNA 
      content, respectively, it is actually a measure of the fluorescence of dye bound 
      to nucleic acids, and may vary with changes in accessibility to the dye. It has 
      been shown for example that extraction of nuclear proteins results in a marked 
      increase in DNA stainability. Moreover, in certain cell systems the binding of 
      fluorochromes correlates with structural modifications in chromatin that 
      accompany cell differentiation. We report here that changes in green & red 
      fluorescence intensity also occur in long-term monocyte cultures. The increased 
      red fluorescence intensity observed in cultured monocytes may reflect ribosomal 
      RNA synthesis and the increased green fluorescence enhanced AO accessibility to 
      DNA due to changes in chromatin organization. We compared cultured monocytes from 
      bladder cancer patients and healthy donors. The results indicate a small but 
      statistically significantly greater increase in mean green & red fluorescence of 
      cultured monocytes from the cancer patients. These fluorescence variations may 
      indicate differences in the immunologic status of cancer patients and/or be 
      related to disease state.
FAU - Myc, A
AU  - Myc A
AD  - Department of Immunochemistry, Memorial Sloan-Kettering Cancer Center, New York, 
      New York 10021.
FAU - Pizzolo, J G
AU  - Pizzolo JG
FAU - Dygulski, K
AU  - Dygulski K
FAU - Melamed, M R
AU  - Melamed MR
LA  - eng
GR  - R0 1-CA-14134/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - 0 (Chromatin)
RN  - 0 (Indoles)
RN  - 47165-04-8 (DAPI)
RN  - 9007-49-2 (DNA)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - *Acridine Orange
MH  - Cells, Cultured
MH  - Chromatin
MH  - Culture Techniques/instrumentation
MH  - DNA/analysis
MH  - *Flow Cytometry
MH  - Fluorescence
MH  - Humans
MH  - Indoles
MH  - Monocytes/*chemistry
MH  - Urinary Bladder Neoplasms/blood
EDAT- 1992/01/01 00:00
MHDA- 1992/01/01 00:01
CRDT- 1992/01/01 00:00
PHST- 1992/01/01 00:00 [pubmed]
PHST- 1992/01/01 00:01 [medline]
PHST- 1992/01/01 00:00 [entrez]
AID - 10.1002/cyto.990130115 [doi]
PST - ppublish
SO  - Cytometry. 1992;13(1):103-7. doi: 10.1002/cyto.990130115.