PMID- 15477505
OWN - NLM
STAT- MEDLINE
DCOM- 20041203
LR  - 20061115
IS  - 0003-9942 (Print)
IS  - 0003-9942 (Linking)
VI  - 61
IP  - 10
DP  - 2004 Oct
TI  - Relapsing-remitting multiple sclerosis and human herpesvirus 6 active infection.
PG  - 1523-7
AB  - BACKGROUND: Recent studies have focused on the relationship between human 
      herpesvirus 6 (HHV-6) and multiple sclerosis (MS). OBJECTIVE: To analyze HHV-6 
      messenger RNA expression in patients with relapsing-remitting (RR) MS vs healthy 
      blood donors (HBDs). DESIGN: One hundred fifty-four subjects were enrolled in the 
      study: 105 patients with RRMS (32 in relapse) and 49 HBDs. Total DNA and 
      messenger RNA were extracted from serum and blood samples, respectively, and 
      analyzed by quantitative real-time reverse transcription-polymerase chain 
      reaction for the detection of 3 HHV-6 immediate-early genes (U16/U17,U89/U90, and 
      U94) and both HHV-6 variants (HHV-6A and HHV-6B). RESULTS: Active HHV-6 infection 
      was detected in 16% of patients with RRMS vs 0% of HBDs (P = .003). Seven 
      patients with RRMS with exacerbation had HHV-6 active replication, and the virus 
      remained latent in only 1 of them. We did not find any statistically significant 
      difference between HHV-6 active or latent infection for patients in remission (P 
      = .12). Among patients with RRMS with HHV-6 active replication, viral load was 
      higher when they experienced an acute attack than when in remission (P = .04). In 
      those patients with RRMS who had an active infection only, HHV-6A was found. 
      Cell-free HHV-6 DNA detected in serum samples confirmed the results. CONCLUSIONS: 
      The results show that a subset of patients with RRMS experience HHV-6 active 
      infection, and there likely is an association between the viral active 
      replication and relapses; therefore, HHV-6 active infection may imply a greater 
      risk of exacerbations in a subgroup of patients with RRMS.
FAU - Alvarez-Lafuente, Roberto
AU  - Alvarez-Lafuente R
AD  - Servicio de Neurologia, Hospital Clinico San Carlos, 28040 Madrid, Spain. 
      labesmul@hcsc.es
FAU - De las Heras, Virginia
AU  - De las Heras V
FAU - Bartolome, Manuel
AU  - Bartolome M
FAU - Picazo, Juan Jose
AU  - Picazo JJ
FAU - Arroyo, Rafael
AU  - Arroyo R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arch Neurol
JT  - Archives of neurology
JID - 0372436
RN  - 0 (DNA, Viral)
RN  - 0 (RNA, Messenger)
SB  - IM
CIN - Arch Neurol. 2004 Oct;61(10):1500-2. PMID: 15477500
MH  - Adult
MH  - DNA, Viral/blood
MH  - Female
MH  - Herpesviridae Infections/blood/complications/*virology
MH  - Herpesvirus 6, Human/genetics/*isolation & purification
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Multiple Sclerosis, Relapsing-Remitting/blood/complications/*virology
MH  - RNA, Messenger/biosynthesis
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - Viral Load/statistics & numerical data
EDAT- 2004/10/13 09:00
MHDA- 2004/12/16 09:00
CRDT- 2004/10/13 09:00
PHST- 2004/10/13 09:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/10/13 09:00 [entrez]
AID - 61/10/1523 [pii]
AID - 10.1001/archneur.61.10.1523 [doi]
PST - ppublish
SO  - Arch Neurol. 2004 Oct;61(10):1523-7. doi: 10.1001/archneur.61.10.1523.