PMID- 15478372
OWN - NLM
STAT- MEDLINE
DCOM- 20041116
LR  - 20220408
IS  - 1547-5654 (Print)
IS  - 1547-5646 (Linking)
VI  - 1
IP  - 3
DP  - 2004 Oct
TI  - Minimally invasive delivery of stem cells for spinal cord injury: advantages of 
      the lumbar puncture technique.
PG  - 330-7
AB  - OBJECT: Stem cell therapy has been shown to have considerable therapeutic 
      potential for spinal cord injuries (SCIs); however, most experiments in animals 
      have been performed by injecting cells directly into the injured parenchyma. This 
      invasive technique compromises the injured spinal cord, although it delivers 
      cells into the hostile environment of the acutely injured cord. In this study, 
      the authors tested the possibility of delivering stem cells to injured spinal 
      cord by using three different minimally invasive techniques. METHODS: Bone marrow 
      stromal cells (BMSCs) are clinically attractive because they have shown 
      therapeutic potential in SCI and can be obtained in patients at the bedside, 
      raising the possibility of autologous transplantation. In this study 
      transgenically labeled cells were used for transplantation, facilitating 
      posttransplantation tracking. Inbred Fisher-344 rats received partial cervical 
      hemisection injury, and 2 x 10(6) BMSCs were intravenously, intraventricularly, 
      or intrathecally transplanted 24 hours later via lumbar puncture (LP). The 
      animals were killed 3, 10, or 14 days posttransplantation, and tissue samples 
      were submitted to histochemical and immunofluorescence analyses. For additional 
      comparison and validation, lineage restricted neural precursor (LRNP) cells 
      obtained from E13.5 rat embryos were transplanted via LP, and these findings were 
      also analyzed. CONCLUSIONS: Both BMSCs and LRNP cells home toward injured spinal 
      cord tissues. The use of LP and intraventricular routes allows more efficient 
      delivery of cells to the injured cord compared with the intravenous route. Stem 
      cells delivered via LP for treatment of SCI may potentially be applicable in 
      humans after optimal protocols and safety profiles are established in further 
      studies.
FAU - Bakshi, Ajay
AU  - Bakshi A
AD  - Department of Neurobiology and Anatomy, Drexel University College of Medicine, 
      Philadelphia, Pennsylvania 19129, USA. ab99@drexel.edu
FAU - Hunter, Corey
AU  - Hunter C
FAU - Swanger, Sharon
AU  - Swanger S
FAU - Lepore, Angelo
AU  - Lepore A
FAU - Fischer, Itzhak
AU  - Fischer I
LA  - eng
GR  - NS24707/NS/NINDS NIH HHS/United States
GR  - NS37515/NS/NINDS NIH HHS/United States
GR  - NS43882/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosurg Spine
JT  - Journal of neurosurgery. Spine
JID - 101223545
SB  - IM
MH  - Animals
MH  - Female
MH  - *Minimally Invasive Surgical Procedures
MH  - Rats
MH  - Rats, Inbred F344
MH  - Spinal Cord Injuries/pathology/physiopathology/*surgery
MH  - *Spinal Puncture
MH  - Stem Cell Transplantation/*methods
MH  - Stromal Cells/*transplantation
EDAT- 2004/10/14 09:00
MHDA- 2004/11/17 09:00
CRDT- 2004/10/14 09:00
PHST- 2004/10/14 09:00 [pubmed]
PHST- 2004/11/17 09:00 [medline]
PHST- 2004/10/14 09:00 [entrez]
AID - 10.3171/spi.2004.1.3.0330 [doi]
PST - ppublish
SO  - J Neurosurg Spine. 2004 Oct;1(3):330-7. doi: 10.3171/spi.2004.1.3.0330.