PMID- 15478612
OWN - NLM
STAT- MEDLINE
DCOM- 20041028
LR  - 20061115
IS  - 0379-4172 (Print)
IS  - 0379-4172 (Linking)
VI  - 31
IP  - 5
DP  - 2004 May
TI  - [Cloning and analysis of six full-length cDNA similar to sheep KAP6-1 from 
      cashmere goat].
PG  - 502-7
AB  - Cashmere is the fiber of kings, produced from the cashmere goat (Capra hircus). 
      Cashmere fabric has little squama. Due to its lightness cashmere feels smooth and 
      silky. An intriguing feature of cashmere structure is multiplicity of the hair 
      keratin proteins with distinctive amino acid compositions. To study the role and 
      regulation of one of these keratins, we used the SMART ( switching mechanism at 
      5' end of RNA transcript) technology to construct a cDNA library of skin tissue 
      from an Inner Mongolia male cashmere goat. A total of 636 cDNA sequences were 
      obtained by randomly sequenced from 5' of cDNA library. Sequence comparison with 
      the GenBank database confirmed that there are 41 sequences showed high nucleotide 
      sequence identity in the coding region with sheep keratin associated protein 6-1 
      (KAP6-1). They represent six different cDNAs (The accession numbers in GenBenk 
      are AY310749, AY310750, AY310751, AY310752, AY310753 and AY310754, respectively). 
      They are full-length cDNAs according to the known KAP6-1 genomic gene sequences 
      of sheep. From the nucleotide sequences the open reading frames were identified, 
      that encoded six basic proteins of 82, 84, 71, 71, 83, 83 amino acids, 
      respectively, with a combined glycine and tyrosine content of about 60%. Compared 
      analysis showed that the KAPs from goats shared more than 55.4% identities in 
      nucleotide sequences and more than 79.8% identities in amino acid sequences with 
      each other, and shared highest identities (81.9% approximately 98.8%) with KAP6-1 
      from sheep. The KAP6s from different animal species shared more than 50% 
      identities in amino acid sequences with each other.
FAU - Yin, Jun
AU  - Yin J
AD  - College of Biotechnology, Inner Mongolia Agricultural University, Huhhot 010018, 
      China.
FAU - Hu, Ting-Mao
AU  - Hu TM
FAU - Li, Jin-Quan
AU  - Li JQ
FAU - Zhang, Chun-Lan
AU  - Zhang CL
FAU - Guo, Zhi-Cheng
AU  - Guo ZC
FAU - Zhou, Huan-Min
AU  - Zhou HM
LA  - chi
SI  - GENBANK/AY310749
SI  - GENBANK/AY310750
SI  - GENBANK/AY310751
SI  - GENBANK/AY310752
SI  - GENBANK/AY310753
SI  - GENBANK/AY310754
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Yi Chuan Xue Bao
JT  - Yi chuan xue bao = Acta genetica Sinica
JID - 7900784
RN  - 0 (DNA, Complementary)
RN  - 68238-35-7 (Keratins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - DNA, Complementary/*chemistry
MH  - Goats/*genetics
MH  - Keratins/chemistry/*genetics
MH  - Male
MH  - Molecular Sequence Data
MH  - Sequence Homology, Amino Acid
MH  - Sheep/*genetics
EDAT- 2004/10/14 09:00
MHDA- 2004/10/29 09:00
CRDT- 2004/10/14 09:00
PHST- 2004/10/14 09:00 [pubmed]
PHST- 2004/10/29 09:00 [medline]
PHST- 2004/10/14 09:00 [entrez]
PST - ppublish
SO  - Yi Chuan Xue Bao. 2004 May;31(5):502-7.