PMID- 15478948
OWN - NLM
STAT- MEDLINE
DCOM- 20050204
LR  - 20191109
IS  - 0148-0545 (Print)
IS  - 0148-0545 (Linking)
VI  - 27
IP  - 3
DP  - 2004 Aug
TI  - Effects of BSO and L-cysteine on drug-induced cytotoxicity in primary cell 
      cultures: drug-, cell type-, and species-specific difference.
PG  - 269-80
AB  - The effects of DL-buthionine-(S,R)-sulfoximine(BSO) and L-cysteine(CYS) on 
      cytotoxicity induced by cisplatin(CP) and diclofenac(DIC) in primary cell 
      cultures of hepatocytes and renal tubular epithelial cells(RTEC) isolated from 
      rats or monkeys were studied. Hepatocytes and RTEC were inoculated into 
      collagen-coated 96-well culture plates. After preincubation, a series of 
      concentrations of CP or DIC were added, and 16 h and 4 h prior to CP and DIC, 40 
      microM BSO and 5 mM CYS were added, respectively. MTT assays were performed to 
      evaluate cytotoxicity(concentrations of drug that inhibited 50% cell growth, 
      IC50). CYS made IC50s of CP in rat and monkey RTEC increase up to more than 5 mM, 
      but BSO made IC50s of CP in rat RTEC lower down with bigger magnitude than that 
      in monkey RTEC; similarly, CYS made IC50s of CP in rat hepatocytes increase up to 
      more than 5 mM, but BSO made IC50s lower down with bigger magnitude than that in 
      rat RTEC. However, neither CYS nor BSO had significant effects on all IC50s of 
      DIC in all examined cells. These results suggested that during CP-induced stress 
      state, rat hepatocytes were more susceptible to changes of GSH level than rat 
      RTEC, and rat RTEC were more dependent on intracellular GSH status than monkey 
      RTEC. DIC-induced cytotoxicity in RTEC and hepatocytes is independent of GSH 
      level.
FAU - Lu, Yongke
AU  - Lu Y
AD  - Department of Toxicology, Dalian Medical University, Dalian, China. 
      luyongke@yahoo.com
FAU - Kawashima, Akira
AU  - Kawashima A
FAU - Horii, Ikuo
AU  - Horii I
FAU - Zhong, Laifu
AU  - Zhong L
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Drug Chem Toxicol
JT  - Drug and chemical toxicology
JID - 7801723
RN  - 0 (Antimetabolites)
RN  - 5072-26-4 (Buthionine Sulfoximine)
RN  - GAN16C9B8O (Glutathione)
RN  - K848JZ4886 (Cysteine)
SB  - IM
MH  - Animals
MH  - Antimetabolites/*pharmacology
MH  - Buthionine Sulfoximine/*pharmacology
MH  - Cell Survival/*drug effects
MH  - Cells, Cultured
MH  - Cysteine/*pharmacology
MH  - Epithelial Cells/drug effects
MH  - Glutathione/metabolism
MH  - Hepatocytes/drug effects
MH  - Macaca fascicularis
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Species Specificity
EDAT- 2004/10/14 09:00
MHDA- 2005/02/05 09:00
CRDT- 2004/10/14 09:00
PHST- 2004/10/14 09:00 [pubmed]
PHST- 2005/02/05 09:00 [medline]
PHST- 2004/10/14 09:00 [entrez]
AID - 10.1081/dct-120037748 [doi]
PST - ppublish
SO  - Drug Chem Toxicol. 2004 Aug;27(3):269-80. doi: 10.1081/dct-120037748.