PMID- 1547912
OWN - NLM
STAT- MEDLINE
DCOM- 19920423
LR  - 20190813
IS  - 0303-7207 (Print)
IS  - 0303-7207 (Linking)
VI  - 83
IP  - 2-3
DP  - 1992 Feb
TI  - The role of specific germ cell types in modulation of the secretion of 
      androgen-regulated proteins (ARPs) by stage VI-VIII seminiferous tubules from the 
      adult rat.
PG  - 219-31
AB  - This study has assessed the role of specific germ cell types in modulating the 
      secretion of seven previously identified androgen-regulated proteins (ARPs) as 
      well as the increase in overall level of protein secretion by seminiferous 
      tubules (ST) at stages VI-VIII of the spermatogenic cycle in the rat. 
      Methoxyacetic acid (MAA) was administered at 650 mg/kg to induce 80-100% 
      depletion of pachytene and later spermatocytes; ST at stages VI-VIII were then 
      isolated at 4, 18 and 30 days after MAA treatment when pachytene spermatocytes 
      (PS), round spermatids (RS) or elongate spermatids (ES), respectively, were 
      depleted selectively. By comparison with ST isolated from controls or from rats 4 
      days after ethanedimethane sulphonate (EDS)-induced testosterone withdrawal, the 
      overall level of incorporation of 35S-methionine into ST-secreted proteins was 
      assessed after culture for 22 h at 32 degrees C as well as the relative abundance 
      of ARPs identified by two-dimensional sodium dodecyl sulphate-polyacrylamide gel 
      electrophoresis (SDS-PAGE). Depletion of PS caused a substantial decrease in 
      abundance of ARP-6 (53-57 kDa; pI 5.7-5.8) and ARP-7 (56-59 kDa; pI 5.9-6.2) and 
      minor and more variable decreases in ARP-3 (27-30 kDa; pI 7.4) and ARP-4 (38 kDa; 
      pI 6.0-6.2); ARP-6 and ARP-7 are comparable in size to the A and B forms of 
      P-Mod-S, a product of the peritubular cells. Depletion of RS resulted in the 
      complete disappearance of ARP-2 (13-14.5 kDa; pI 7.3-7.6), a decrease in 
      abundance of ARP-7 and variable decreases in ARP-1 (approximately 9.5 kDa; pI 
      6.9-7.4) and ARP-6; ARP-1 and -2 are thought to be Sertoli cell-secreted 
      proteins. Depletion of ES had no effect on secretion of the ARPs. Depletion of 
      PS, RS or ES resulted in significant (P less than 0.001) decreases of 26, 38 and 
      42% respectively in the overall level of incorporation of methionine into 
      ST-secreted proteins, but this was significantly less than the decrease (55%) 
      induced by EDS-induced testosterone withdrawal. These results show that the 
      androgen-dependent changes in secretion of proteins, which occur specifically at 
      stages VI-VIII of the normal spermatogenic cycle, are largely, if not completely, 
      dependent on a normal germ cell complement, with each germ cell type regulating 
      the secretion of proteins essential for its own development.(ABSTRACT TRUNCATED 
      AT 400 WORDS)
FAU - McKinnell, C
AU  - McKinnell C
AD  - MRC Reproductive Biology Unit, Centre for Reproductive Biology, Edinburgh, 
      Scotland, UK.
FAU - Sharpe, R M
AU  - Sharpe RM
LA  - eng
PT  - Journal Article
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (Acetates)
RN  - 0 (Proteins)
RN  - 3XMK78S47O (Testosterone)
RN  - F11T1H7Q7W (methoxyacetic acid)
SB  - IM
MH  - Acetates/pharmacology
MH  - Animals
MH  - Cell Division/drug effects/physiology
MH  - Culture Techniques
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Male
MH  - Proteins/*metabolism
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Seminiferous Tubules/cytology/*metabolism
MH  - Spermatids/*physiology
MH  - Spermatocytes/*physiology
MH  - *Spermatogenesis
MH  - Testosterone/*physiology
EDAT- 1992/02/01 00:00
MHDA- 1992/02/01 00:01
CRDT- 1992/02/01 00:00
PHST- 1992/02/01 00:00 [pubmed]
PHST- 1992/02/01 00:01 [medline]
PHST- 1992/02/01 00:00 [entrez]
AID - 0303-7207(92)90162-Y [pii]
AID - 10.1016/0303-7207(92)90162-y [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 1992 Feb;83(2-3):219-31. doi: 10.1016/0303-7207(92)90162-y.