PMID- 1548046
OWN - NLM
STAT- MEDLINE
DCOM- 19920423
LR  - 20191021
IS  - 0882-0139 (Print)
IS  - 0882-0139 (Linking)
VI  - 21
IP  - 1
DP  - 1992 Feb
TI  - A sensitive ELISA for the detection of human monocyte chemoattractant protein-1 
      (MCP-1).
PG  - 39-45
AB  - The recruitment of monocytes into tissue is associated with both acute and 
      chronic inflammation. Although monocyte migration is measured in vitro by 
      monocyte chemotaxis, this technique is often difficult to determine the specific 
      quantitative contribution of a monocyte chemotaxin. We have developed a sensitive 
      sandwich ELISA for the detection of monocyte chemoattractant protein-1 (MCP-1), a 
      highly specific monocyte activating/chemotactic peptide. Polyclonal antibodies 
      were generated from rabbits. The IgG fraction of the antiserum was isolated by a 
      protein A column, with a portion of the antibodies biotinylated. 
      Avidin-conjugated horse radish peroxidase was used for enzymatic, colorimetric 
      analysis. The lower threshold for detection of MCP-1 was 50 pg/ml, and the ELISA 
      was specific for MCP-1, since it failed to recognize other cytokines in a 
      dose-dependent fashion. Furthermore, this ELISA had the capacity to measure 
      endothelial cell and pulmonary fibroblast-derived MCP-1. The development of a 
      sensitive ELISA for the detection MCP-1 is significant, since it will allow the 
      measurement MCP-1 from biologically relevant fluids, and aid in establishing 
      whether MCP-1 is present in disease.
FAU - Evanoff, H L
AU  - Evanoff HL
AD  - Department of Pathology, University of Michigan Medical School, Ann Arbor 
      48109-0360.
FAU - Burdick, M D
AU  - Burdick MD
FAU - Moore, S A
AU  - Moore SA
FAU - Kunkel, S L
AU  - Kunkel SL
FAU - Strieter, R M
AU  - Strieter RM
LA  - eng
GR  - HL02401/HL/NHLBI NIH HHS/United States
GR  - HL31693/HL/NHLBI NIH HHS/United States
GR  - HL35276/HL/NHLBI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Immunol Invest
JT  - Immunological investigations
JID - 8504629
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Culture Media)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2
MH  - Chemotactic Factors/*analysis
MH  - Culture Media
MH  - Endothelium, Vascular/metabolism
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - Fibroblasts/metabolism
MH  - Humans
MH  - Monocytes/metabolism
MH  - Neutrophils/metabolism
MH  - Rabbits
MH  - Recombinant Proteins
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
EDAT- 1992/02/01 00:00
MHDA- 1992/02/01 00:01
CRDT- 1992/02/01 00:00
PHST- 1992/02/01 00:00 [pubmed]
PHST- 1992/02/01 00:01 [medline]
PHST- 1992/02/01 00:00 [entrez]
AID - 10.3109/08820139209069361 [doi]
PST - ppublish
SO  - Immunol Invest. 1992 Feb;21(1):39-45. doi: 10.3109/08820139209069361.